Select-HA Hyaluronic Acid

Hyaluronan (HA; hyaluronic acid) is a member of the glycosaminoglycan class of natural polysaccharides. Hyaluronan is a linear, unbranched polymer composed of alternating residues of β-D-(1→3) glucuronic acid (GlcA) and β-D-(1→4)-N-acetylglucosamine (GlcNAc). Historically hyaluronan has been isolated from animal sources or extracted from bacteria, including strains of Staphyloccus. In animals and bacteria, hyaluronan has a molecular mass from 104 to 107 Dalton, with the typical polydispersity (Mw/Mn) of commercially available hyaluronan >1.5. Hyaluronan is a major component of the extracellular matrix but HA is also associated with multiple biological roles including modulation of cellular adhesion, signaling, wound healing, cellular regeneration, inflammation, and cancer.1-3

Unlike other glycosaminoglycans, hyaluronan does not attach to proteins to form proteoglycans and it is not sulfated. The biological functionality of hyaluronan appear to be dependant on polymer size, with smaller polymers and oligosaccharides having contradictory properties.1 Native hyaluronans are polymeric mixtures and may contain additional contaminants, requiring researchers to perform extensive sample degradation and chromatographic purification to obtain polymers of specific size or hyaluronan oligomers.

References
Composed of alternating residues of β-D-(1→3) glucuronic acid and β-D-(1→4)-

Oligo-HA and nanoHA

Oligo-HA and nanoHA are hyaluronan oligosaccharides designed for research use. The Oligo-HA products are small-molecular weight hyaluronic acid prepared with streptococcal or recombinant bacterial HA via testicular hyaluronidase digestion and purified by gel filtration. The hyaluronan disaccharide subunit is glucuronic acid-β-D-(1→3)-N-acetyl-D-glucosamine-β-D-(1→4) (GlcAβ3GlcNAcβ4), and each Oligo-HA is a defined oligomer of the subunit.

The nanoHA products are small-molecular weight hyaluronic acid oligomers prepared by chemoenzymatic synthesis using immobilized enzymes.6 The name of each nanoHA indicates the number of monosaccharide units in the oligomer. The reducing end contains N-acetyl-D-glucosamine.

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N9164 nanoHA5 low endotoxin
N9039 nanoHA7 low endotoxin
O9140 Oligo-HA4 low endotoxin
O9015 Oligo-HA6 low endotoxin

Select-HA by Size Distribution

Select-HA is a monodisperse hyaluronic acid prepared through patented enzymatic synthesis where a very high level of size control is possible.4,5 Each Select-HA is a narrow-size distribution HA with a specific average molecular weight within a defined range. Polydispersity of Select-HA is as low as 1.02 and averages 1.1 (an ideal monodisperse polymer has a polydispersity value of 1).

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Description

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S0451 Select-HA Hyaluronan 50K, mol wt 25-75 kDa
S0326 Select-HA Hyaluronan 50K, mol wt 25-75 kDa, low endotoxin
S0201 Select-HA Hyaluronan 150K, mol wt 125-175 kDa
S9950 Select-HA Hyaluronan 500K, mol wt 400-600 kDa