CleanAmp dNTPs are a new solution for Hot-Start PCR. These modified dNTPs help to control mis-priming and primer dimer formation by blocking DNA polymerase nucleotide incorporation until elevated temperatures are achieved. These modified nucleoside triphosphates are activated by the elevated temperatures of PCR thermal cycling.
CleanAmp dNTPs are modified with a thermolabile protecting group (see figure below) at the 3′ terminus. The presence of this modification blocks DNA polymerase nucleotide incorporation until the nucleotide protecting group is removed using a heat activation step. When standard cycling protocols are employed, a 0-10 minute initial denaturation step at 95°C allows for robust amplification. For faster thermal cycling protocols, an initial denaturation is not required. In many cases, all that is needed to successfully utilize CleanAmp dNTPs in a PCR reaction is to replace the natural nucleotides with CleanAmp dNTPs.
CleanAmp™ Products, technology and their use may be covered by one or more patents or pending patent applications. For more information, please visit the product detail pages at sigmaaldrich.com.