CYP450 Products

  • The cytochrome P450 (CYP450) family of enzymes is important in drug metabolism and biotransformation.
  • The CYP450 kits utilize MS, specifically MRM methodology, to monitor for the presence of up to 7 specific CYP450 isoforms, and for quantification based on precise peptide standards for those isoforms.
  • These MS-based kits offer alternatives to protocols based on measurement of mRNA levels, enzyme activity, or Western blotting.
  • Related Documents:
Protocol for the CYP450 Protein Assay - Human Induction Kit
Quick Reference Card for the CYP450 Protein Assay - Human Induction Kit
Technical Note on the use of the CYP450 Protein Assay - Human Induction Kit
Protocol for the CYP450 Protein Assay - Human Induction Kit Extended Panel
Technical Note on the use of the CYP450 Protein Assay - Human Induction Kit Extended Panel

References
1. Williamson, B.L., et al., Quantitative protein determination for CYP induction via LC-MS/MS. Proteomics, 11(1), 33-41 (2011). Abstract
2. Li, X., et al., Characterization of T-5 N-Oxide Formation as the First Highly Selective Measure of CYP3A5 Activity. Drug Metab. Dispos., 42(3), 334-342 (2014). Abstract
3. Xu, L., et al., The use of a rapid MS-based method for the quantification of the CYP 3A4 protein directly from hepatocyte cell lysate for CYP induction studies. Bioanalysis, 6(24), 3271-3282 (2014). Publication

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  4465863 CYP450 Extended Panel - 100 Assay Kit The SCIEX iChemistry® CYP450 Peptide Standards Extended Panel Human Induction 100 Assay Kit provides a complete protocol, instrument data acquisition methods, processing methods and stable isotope-labeled synthetic peptide standards for the analysis of seven human CYP450 isoforms (1A2, 2B6, 2C9, 2C19, 2E1, 3A4, 3A5) for induction studies using LC/MS/MS.  Kit contains enough reagents for 100 assays.  Trypsin TPCK (500 μg) and Peptide C18 Column must be ordered separately.
  4466004 CYP450 Extended Panel - Starter Kit The SCIEX iChemistry® CYP450 Peptide Standards Extended Panel Human Induction Starter Kit provides a complete package for the analysis of seven human CYP450 isoforms (1A2, 2B6, 2C9, 2C19, 2E1, 3A4, 3A5) using MRM analysis. The kit contains a detailed protocol, instrument data acquisition methods, processing methods, LC column, stable isotope-labeled synthetic peptide standards, trypsin, and all buffers and reagents for 100 assays.
  4445252 P450 Human Induction 100 Assay Kit The P450 Human Induction 100 Assay Kit provides a complete protocol, instrument data acquisition methods, processing methods and stable isotope labeled synthetic peptide standards for the analysis of four human CYP450 isoforms (1A2, 2B6, 3A4, and 3A5) for induction studies using LC/MS/MS. Kit contains enough reagents for 100 assays. Must order Trypsin TPCK (500 μg) and Peptide C18 Column separately.
  4445494 P450 Human Induction Starter Kit The P450 Human Induction Starter Kit provides a complete package for the analysis of four human CYP450 isoforms (1A2, 2B6, 3A4, and 3A5) using MRM analysis. The kit contains a detailed protocol, instrument data acquisition methods, processing methods, LC column, stable isotope-labeled synthetic peptide standards, trypsin, and all buffers and reagents for 100 assays.