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iTRAQ® Reagents

  • The iTRAQ® (Isobaric tags for Relative and Absolute Quantification) quantitative proteomics reagents derivatize amine groups of proteins and incorporate stable isotopes into the isobaric tag portion of the reagents.
  • These isobaric tags provide specific reporter ions, upon fragmentation, which are specifically monitored in MS analysis.
  • 8 different isobaric reagents are available, with the following reporter ions / isobaric tag values: 113 / 192, 114 / 191, 115 / 190, 116 / 189, 117 / 188, 118 / 187, 119 / 186, and 121 / 184.
  • Thus multiple samples, each with a different isobaric tag, can be combined, or “multiplexed” into a single sample for simultaneous MS analysis.
  • Related Documents:
Protocol for iTRAQ Reagents
Quick Reference Card for iTRAQ Reagents
Application Note: Characterizing the Secreted Proteome of Mycobacterium tuberculosis: Analysis of Sub-Cellular Proteomes
Application Note: Protein Biomarker Research Pipeline for Developing Protein Biomarkers for Diabetic Kidney Disease

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  4352157 Trypsin, TPCK-Treated Trypsin is a serine protease that specifically hydrolyzes peptide bonds at the carboxyl side of Lysine and Arginine residues. This modified trypsin has been treated with N-tosyl-L-phenylalanine chloromethyl ketone (TPCK) to inactivate extraneous chymotryptic activity. This trypsin product also contains calcium chloride, which may improve consistency in digestion. Each package contains 10 vials, with 25 μg in each vial. 
  4370285 Trypsin, TPCK-Treated Trypsin is a serine protease that specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. This modified trypsin has been treated with N-tosyl-L-phenylalanine chloromethyl ketone (TPCK) to inactivate extraneous chymotryptic activity. Each package contains 8 vials, with 25 μg in each vial.
  4390733 iTRAQ® Reagent - 8PLEX 25U Bulk Pack Includes 8 tubes. Each tube contains 25 units of an individual 8-plex reagent. This kit contains the reagents in the equivalent Sciex iChemistry® product number 4393528.
  4390731 iTRAQ® Reagent - 8PLEX 50U Bulk Pack Includes 8 tubes. Each tube contains 50 units of an individual 8-plex reagent. This product contains the reagents in the equivalent Sciex iChemistry® product 4393529.
  4381663 iTRAQ® Reagent - 8PLEX Multiplex Kit Contains iTRAQ® Reagents 113, 114, 115, 116, 117, 118, 119 and 121 and sufficient material for five 8-plex assays. Multiplex Buffer kit (Cat. No. 4381664) to be ordered separately.

Cat. No. 4381663 contains the equivalent reagents in the Sciex iChemistry® product number 4390812.
  4381662 iTRAQ® Reagent - 8PLEX One Assay Kit Contains iTRAQ® Reagents 113, 114, 115, 116, 117, 118, 119 and 121, and also SDS Denaturant, TCEP Reducing Reagent, MMTS cysteine blocking reagent, TEAB buffer, and Isopropanol, for initial protocol development.

This kit contains the reagents in the equivalent SCIEX iChemistry® product number 4390811.
  4381664 iTRAQ® Reagent - Multiplex Buffer Kit Contains SDS – Denaturant, TCEP Reducing Reagent, MMTS Cysteine Blocking Reagent, TEAB Buffer and Isopropanol, sufficient material for either two 8-plex Multiplex Kits, three 4-plex Kits, or four mTRAQ® 10 Assay Kits.
  4370280 iTRAQ® Reagent Application Kit - Plasma Contains sufficient reaction solutions and iTRAQ® Reagents 114, 115, 116 and 117 for 8 duplex or four 4-plex experiments based on 50 μg of protein per sample.
  4374321 iTRAQ® Reagent Application Kit - Protein Contains sufficient reaction solutions, test mixture and iTRAQ® Reagents 114, 115, 116 and 117 (for 25 μg protein) to label 10 duplex, 6 three-plex or 5 four-plex experiments.
  4369561 iTRAQ® Reagents 3-Assay Duplex Trial Kit Contains iTRAQ® Reagents 114 and 117, cation-exhange buffers, cartridges, reaction solution and test mixture, for initial protocol development.
  4352160 iTRAQ® Reagents Methods Developments Kit Contains iTRAQ® reagents 114, 117, test sample, buffers and reagents for up to three separate analyses. Each individual reagent can label up to 100 μg of protein. Included: Starter Kit - Hardware, Cation Exchange Buffer Pack and Cation Exchange Cartridge, Protocol, iTRAQ® Reagent Kit.
  4352135 iTRAQ® Reagents Multiplex Kit Contains iTRAQ® reagents 114, 115, 116, 117, the appropriate buffers and reagents for ten 2-plex, six 3-plex, or five 4-plex assays. 10-PK trypsin is sold separately. Each individual reagent is capable of labeling up to 100 μg of protein.