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Duolink® using PLA® Technology

Accelerate the Signaling Pathway Discovery Process

Detect, quantify and determine cell localization of a specific protein complex in the same experiment. Duolink, based on in situ PLA, which is a proximity ligation assay, enables you to visualize protein interactions in fixed cells and tissue samples, all while under endogenous protein expression.

  • Visualize individual interactions without having to overexpress proteins
  • Gain high specificity with dual binding of primary antibodies
  • Single molecule sensitivity due to signal amplification
  • Analyze using a standard fluorescence microscope

Read about how Duolink can detect protein-protein interactions not previously detectable using co-IP
Specific interactions between Smad proteins and AP-1 components determine TGF-beta induced breast cancer cell invasion. Sundqvist, A., et al. Oncogene. 27 Aug 2012.

Read about how Duolink can detect single phosporylation events
Proximity ligation assays for isoform-specific Akt activation in breast cancer identify activated Akt1 as a driver of progression. Spears, M., et al. J. Pathol. 227, (2012).



Browse more Duolink references

Duolink Starter Kits

Obtain Comprehensive Protein Analysis with Duolink

Detect, quantify and determine cell localization of protein interactions and their modifications in a single experiment. Duolink, based on in situ PLA, which is a proximity ligation assay, enables this analysis all while under endogenous protein expression.

To perform a complete Duolink in situ experiment you will need two primary antibodies (IHC, ICC or IF validated) that recognize two target epitopes. Additional reagents required include a pair of PLA probes, one PLUS and one MINUS, your choice of Detection Reagents. Additional reagents include Wash Buffers and Mounting Medium.

The Duolink starter kit is a great way to get all components necessary to analyze up to 30 reactions in one single order. Select one from the 6 available below:

Product #

Description

Add to Cart

DUO92101 Duolink® In Situ Red Starter Kit Mouse/Rabbit
DUO92102 Duolink® In Situ Orange Starter Kit Mouse/Rabbit
DUO92103 Duolink® In Situ Red Starter Kit Mouse/Goat
DUO92104 Duolink® In Situ Orange Starter Kit Mouse/Goat
DUO92105 Duolink® In Situ Red Starter Kit Goat/Rabbit
DUO92106 Duolink® In Situ Orange Starter Kit Goat/Rabbit

Duolink PLA Accessories

Product #

Description

Add to Cart

DUO80102 Duolink® In Situ Brightfield Mounting Medium
DUO82040 Duolink® In Situ Mounting Medium with DAPI
DUO82047 Duolink® In Situ Wash Buffer, Brightfield
DUO82049 Duolink® In Situ Wash Buffers, Fluorescence
DUO82064 Duolink® In Situ Microplate Nuclear Stain, Anti-Fade
DUO82065 Duolink® In Situ Microplate Heat Transfer Block
DUO90806 Duolink® ImageTool

Duolink PLA Detection Reagents

Product #

Description

Add to Cart

DUO92007 Duolink® In Situ Detection Reagents Orange
DUO92008 Duolink® In Situ Detection Reagents Red
DUO92012 Duolink® In Situ Detection Reagents Brightfield
DUO92013 Duolink® In Situ Detection Reagents FarRed
DUO92014 Duolink® In Situ Detection Reagents Green

Duolink PLA Probes

Product #

Description

Add to Cart

DUO92001 Duolink® In Situ PLA Probe Anti-Mouse PLUS Affinity purified Donkey anti-Mouse IgG (H+L)
DUO92002 Duolink® In Situ PLA® Probe Anti-Rabbit PLUS Affinity purified Donkey anti-Rabbit IgG (H+L)
DUO92003 Duolink® In Situ PLA® Probe Anti-Goat PLUS Affinity purified Donkey anti-Goat IgG (H+L)
DUO92004 Duolink® In Situ PLA® Probe Anti-Mouse MINUS Affinity purified Donkey anti-Mouse IgG (H+L)
DUO92005 Duolink® In Situ PLA® Probe Anti-Rabbit MINUS Affinity purified Donkey anti-Rabbit IgG (H+L)
DUO92006 Duolink® In Situ PLA® Probe Anti-Goat MINUS Affinity purified Donkey anti-Goat IgG (H+L)
DUO92009 Duolink® In Situ Probemaker PLUS
DUO92010 Duolink® In Situ Probemaker MINUS