Whole Genome and Transcriptome Amplification

Whole Genome Amplification

The GenomePlex® Whole Genome Amplification (WGA) family of products provides a robust and accurate method of amplifying nanogram quantities of starting material into microgram yields with minimal allele drop out.
The GenomePlex WGA product line utilizes a proprietary amplification technology based upon random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable OmniPlex® Library molecules flanked by universal priming sites. The OmniPlex library is then PCR amplified using universal oligonucleotide primers and a limited number of cycles.
WGA has been used in a variety of applications,1, 2, 3 and is suitable for use with purified genomic DNA from a variety of sources. GenomePlex WGA uses nanogram quantities of starting genomic DNA, which after PCR yields 5 to 10 μg of WGA product. After purification, the WGA product can be analyzed in a manner similar to any genomic or chromosomal DNA sample.

  • Choose from a variety of DNA sources: whole blood, buccal swab, blood card, plant, soil and formalin-fixed paraffin-embedded (FFPE) tissue
  • See a complete representation of the entire genome with no detectable allele bias.
  • Increase amplification accuracy with WGA DNA Polymerase
  • Preserve precious source material by amplifying nanogram amounts of starting genomic DNA into microgram yields (on average up to 500fold) in less than three hours
  • Enjoy the compatibility GenomePlex provides with many downstream applications: TaqMan® assays, CGH analysis, SNP analysis, sequencing, or store at 20 °C for future use.

References:
1. Little, S.E., et al., Array CGH using whole genome amplification of fresh-frozen and formalin-fixed, paraffin-embedded tumor DNA. Genomics. Oct 31, 2005.
2. Barker, D. L., et al., Two methods of whole-genome amplification enable accurate genotyping across a 2320-SNP linkage panel. Genome Res., 14, 901-907 (2004).
3. Gribble, S., et al., Chromosome paints from single copies of chromosomes. Chromosome Res., 12, 143-151 (2004).
4. Thorstenson, Y. R., et al., An Automated Hydrodynamic Process for Controlled, Unbiased DNA Shearing. Genome Res., 8, 848-855 (1998).
5. Park, J.W., et al., Comparing Whole-Genome Amplification Methods and Sources of Biological Samples for Single-Nucleotide Polymorphism Genotyping. Clinical Chemistry., 51(8), 1520-1523 (2004).

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GE25-6600-20 Genomiphi Hy Kit GE Healthcare, 25-6600-20, sufficient for 100 reactions
GE25-6600-30 GenomiPhi V2 DNA Amplification Kit, 25 RXN GE Healthcare, 25-6600-30, sufficient for 25 reactions
GE25-6600-31 Genomiphi V2 DNA Amplification Kit GE Healthcare, 25-6600-31, sufficient for 100 reactions
GE25-6600-32 Genomiphi V2 DNA Amplification Kit GE Healthcare, 25-6600-32, sufficient for 500 reactions
GE25-6601-24 RTG GenomiPhi V3 DNA Amplification Kit, 24 RXN GE Healthcare, 25-6601-24, sufficient for 24 reactions
GE25-6601-97 RTG GenomiPhi V3 DNA Amplification Kit, 480 RXN GE Healthcare, 25-6601-97, sufficient for 480 reactions
GE25-6603-24 RTG GenomiPhi HY DNA Amplification Kit, 24 RXN GE Healthcare, 25-6603-24, sufficient for 24 reactions
GE25-6603-97 RTG GenomiPhi HY DNA Amplification Kit, 480 RXN GE Healthcare, 25-6603-97, sufficient for 480 reactions
WGA1 GenomePlex® Whole Genome Amplification (WGA) Kit Kit for whole genome amplification from a variety of DNA sources including FFPE tissue
WGA2 GenomePlex® Complete Whole Genome Amplification (WGA) Kit Optimized kit with enzyme for amplifying a variety of DNA including FFPE tissue
WGA3 GenomePlex® WGA Reamplification Kit Reamplification of WGA product with minimal bias
WGA4 GenomePlex® Single Cell Whole Genome Amplification Kit Amplify genome of a single cell

Whole Transcriptome Amplification

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WTA2 Complete Whole Transcriptome Amplification Kit DNA polymerase included, Complete Kit with optimized enzyme to amplify total RNA in <4 hours, no 3′ bias
SEQR SeqPlex RNA Amplification Kit For use with high throughput sequencing technologies
WTA1 TransPlex® Whole Transcriptome Amplification Kit DNA polymerase separate.

SeqPlex for Next Gen Sequencing

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SEQR SeqPlex RNA Amplification Kit For use with high throughput sequencing technologies
SEQX SeqPlex DNA Amplification Kit For use with high throughput sequencing technologies, Whole Genome Amplification kit designed to facilitate Next Gen Sequencing
SEQXE SeqPlex DNA Amplification Kit For use with high throughput sequencing technologies, Whole Genome Amplification kit designed to facilitate Next Gen Sequencing.