Substrates, Stains and Detection Kits

Colorometric or chromogenic assays allow detection of vector insertion or ligation by converting a substrate (indicator) to a visible chromophore or fluorophore in a reporter gene system, often used in molecular biology during transfection and transformation.

GUS (beta-glucuronidase) staining involves inserting the promoter gene for ß-glucosidase then incubating with a probe such as X-glucoside or X-Glc, which produce a blue chromophore in positive organisms. X-GlcA reacts with plant tissues transfected with the E. coli GUS gene to form an insoluble indigo-blue precipitate. Other indicators produce a magenta, yellow or fluoresce in GUS+ bacterial colonies.

CDP-Star® Chemiluminescent Substrate identifies alkaline phosphatase-labeled molecules in Northern, Southern, and Western blots, on neutral or positively-charged nylon membrane-based applications. Monoclonal Anti-Green Fluorescent Protein (GFP) antibody (mouse) is used for fluorescent monitoring of GFP gene expression.

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B4527 5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside ≥97%
B4532 5-Bromo-6-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt ≥98%
C0712 CDP-Star® Chemiluminescent Substrate 0.25 mM in H2O
GUSS β-Glucuronidase Reporter Gene Staining Kit
G6539 Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse clone GFP-20, ascites fluid
M9130 4-Methylumbelliferyl-β-D-glucuronide hydrate ≥98% (HPLC), BioReagent, for identification of transformed plants
N1627 4-Nitrophenyl β-D-glucuronide ≥98% (TLC)