The GenomePlex
® Whole Genome Amplification (WGA) family of products provides a robust and accurate method of amplifying nanogram quantities of starting material into microgram yields with minimal allele drop out.
The GenomePlex WGA product line utilizes a proprietary amplification technology based upon random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable OmniPlex
® Library molecules flanked by universal priming sites. The OmniPlex library is then PCR amplified using universal oligonucleotide primers and a limited number of cycles.
WGA has been used in a variety of applications,
1, 2, 3 and is suitable for use with purified genomic DNA from a variety of sources. GenomePlex WGA uses nanogram quantities of starting genomic DNA, which after PCR yields 5 to 10 μg of WGA product. After purification, the WGA product can be analyzed in a manner similar to any genomic or chromosomal DNA sample.
- Choose from a variety of DNA sources: whole blood, buccal swab, blood card, plant, soil and formalin-fixed paraffin-embedded (FFPE) tissue
- See a complete representation of the entire genome with no detectable allele bias.
- Increase amplification accuracy with WGA DNA Polymerase
- Preserve precious source material by amplifying nanogram amounts of starting genomic DNA into microgram yields (on average up to 500fold) in less than three hours
- Enjoy the compatibility GenomePlex provides with many downstream applications: TaqMan® assays, CGH analysis, SNP analysis, sequencing, or store at 20 °C for future use.
References:1. Little, S.E.,
et al., Array CGH using whole genome amplification of fresh-frozen and formalin-fixed, paraffin-embedded tumor DNA. Genomics. Oct 31, 2005.
2. Barker, D. L.,
et al., Two methods of whole-genome amplification enable accurate genotyping across a 2320-SNP linkage panel. Genome Res.,
14, 901-907 (2004).
3. Gribble, S.,
et al., Chromosome paints from single copies of chromosomes. Chromosome Res.,
12, 143-151 (2004).
4. Thorstenson, Y. R.,
et al., An Automated Hydrodynamic Process for Controlled, Unbiased DNA Shearing. Genome Res.,
8, 848-855 (1998).
5. Park, J.W.,
et al., Comparing Whole-Genome Amplification Methods and Sources of Biological Samples for Single-Nucleotide Polymorphism Genotyping. Clinical Chemistry.,
51(8), 1520-1523 (2004).
