The GenomePlex® Whole Genome Amplification (WGA) family of products provides a robust and accurate method of amplifying nanogram quantities of starting material into microgram yields with minimal allele drop out. The GenomePlex WGA product line utilizes a proprietary amplification technology based upon random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable OmniPlex® Library molecules flanked by universal priming sites. The OmniPlex library is then PCR amplified using universal oligonucleotide primers and a limited number of cycles. WGA has been used in a variety of applications,1, 2, 3 and is suitable for use with purified genomic DNA from a variety of sources. GenomePlex WGA uses nanogram quantities of starting genomic DNA, which after PCR yields 5 to 10 μg of WGA product. After purification, the WGA product can be analyzed in a manner similar to any genomic or chromosomal DNA sample.
Choose from a variety of DNA sources: whole blood, buccal swab, blood card, plant, soil and formalin-fixed paraffin-embedded (FFPE) tissue
See a complete representation of the entire genome with no detectable allele bias.
Increase amplification accuracy with WGA DNA Polymerase
Preserve precious source material by amplifying nanogram amounts of starting genomic DNA into microgram yields (on average up to 500fold) in less than three hours
Enjoy the compatibility GenomePlex provides with many downstream applications: TaqMan® assays, CGH analysis, SNP analysis, sequencing, or store at 20 °C for future use.
References: 1. Little, S.E., et al., Array CGH using whole genome amplification of fresh-frozen and formalin-fixed, paraffin-embedded tumor DNA. Genomics. Oct 31, 2005. 2. Barker, D. L., et al., Two methods of whole-genome amplification enable accurate genotyping across a 2320-SNP linkage panel. Genome Res., 14, 901-907 (2004). 3. Gribble, S., et al., Chromosome paints from single copies of chromosomes. Chromosome Res., 12, 143-151 (2004). 4. Thorstenson, Y. R., et al., An Automated Hydrodynamic Process for Controlled, Unbiased DNA Shearing. Genome Res., 8, 848-855 (1998). 5. Park, J.W., et al., Comparing Whole-Genome Amplification Methods and Sources of Biological Samples for Single-Nucleotide Polymorphism Genotyping. Clinical Chemistry., 51(8), 1520-1523 (2004).