dNTPs (Deoxynucleotides)

Sigma’s ultrapure deoxynucleotides (dNTPs) are ≥99% pure and are qualified for use in
  • Standard and long PCR
  • Manual and automated sequencing
  • cDNA synthesis
  • Labeling reactions

Individual dNTPs and sets of the four dNTPs are available at 10 mM and 100 mM. The 10 mM mix is ready to use in standard and long PCR.

100 mM Solutions

Product #

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11051440001 dATP Lithium salt
DATP-RO dATP PCR Grade, sodium salt
11051458001 dCTP Lithium salt
DCTP-RO dCTP PCR Grade, sodium salt
D4788 2′-Deoxyadenosine 5′-triphosphate sodium salt solution 100 mM, pH 7
D4913 2′-Deoxycytidine 5′-triphosphate disodium salt 100 mM (pH 7)
D5038 2′-Deoxyguanosine 5′-triphosphate trisodium salt solution 100 mM (pH 7)
DNTP100 Deoxynucleotide Set, 100 mM Individual dNTPs for routine PCR; 0.25 mL each
DNTP100A Deoxynucleotide Set, 100 mM Individual dNTPs for routine PCR; 1 mL each
11051466001 dGTP Lithium salt
DGTP-RO dGTP PCR Grade, sodium salt
11051482001 dTTP Lithium salt
DTTP-RO dTTP PCR Grade, sodium salt
T9656 Thymidine 5′-triphosphate sodium salt solution 100 mM in H2O

Deoxynucleotide Sets

Product #

Description

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DNTPCA2 CleanAmp dNTP Modified dNTP set for hot-start PCR, 2 μmol of each dNTP
DNTPCA10 CleanAmp dNTP 10 μmol of each dNTP, Modified dNTP set for hot-start PCR
DNSTP-RO Deoxynucleoside Triphosphate Set Lithium salt
DNTP-RO Deoxynucleoside Triphosphate Set PCR Grade, sodium salt
DNTP100 Deoxynucleotide Set, 100 mM Individual dNTPs for routine PCR; 0.25 mL each
DNTP100A Deoxynucleotide Set, 100 mM Individual dNTPs for routine PCR; 1 mL each
DNTP10 Deoxynucleotide Set, 10 mM Individual dNTPs for routine PCR; 0.5 mL each

dNTP Mix

Product #

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DNTPCA1 CleanAmp dNTP Hot-start dNTP mix for improved PCR
D7295 Deoxynucleotide Mix, 10 mM Molecular Biology Reagent
11277081001 Hexanucleotide Mix

dUTP and dUTP Mixes

Carryover of previously amplified PCR products is the single most significant source of contamination in subsequent PCR. Several methods have been used to prevent carryover contamination, such as physical containment and chemical treatments.1 One of the most convenient and effective strategies is to include dUTP and uracil DNA glycosylase (UDG) in PCR reactions. In this system, dUTP is used to replace TTP in the PCR reaction so that all PCR products will contain dUTP. Before commencing each PCR, fully assembled reactions are treated with UDG, which cleaves the uracil base in dUTP -containing DNA (amplicon) but does not react with dUTP and has no effect on authentic DNA template containing thymidine bases or RNA. After initial treatment, UDG can be inactivated by heat denaturation prior to the PCR. New PCR product, amplified from authentic DNA template, though containing dUTP, is not destroyed. The degraded DNA is not suitable for use as a hybridization target or as a template for DNA polymerases so cannot be reamplified or serve as a contaminant in subsequent reactions.

Product #

Description

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D0184 2′-Deoxyuridine 5′-triphosphate sodium salt solution 100 mM in H2O
DUTP-RO dUTP PCR Grade, sodium salt
11420470001 dUTP Lithium salt

10 mM Solutions

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Description

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D6920 2′-Deoxyadenosine 5′-triphosphate sodium salt solution 10 mM
D7045 2′-Deoxycytidine 5′-triphosphate disodium salt 10 mM, aqueous solution
D7170 2′-Deoxyguanosine 5′-triphosphate trisodium salt solution 10 mM in H2O
D7295 Deoxynucleotide Mix, 10 mM Molecular Biology Reagent
DNTP10 Deoxynucleotide Set, 10 mM Individual dNTPs for routine PCR; 0.5 mL each
DNTPM-RO PCR Nucleotide Mix
11888412001 PCR Nucleotide MixPLUS
T7791 Thymidine 5′-triphosphate sodium salt solution 10 mM (pH 7.0)