SYBR® Green Based Quantitative PCR

SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. Specificity of Sigma’s SYBR based QPCR detection is greatly enhanced by the incorporation of a hot-start mediated Taq polymerase, JumpStart Taq.
SYBR Green I Based QPCR Technical Animation

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KSPQ12012 KiCqStart® SYBR® Green Primers Predesigned primers for gene expression analysis
KSGA2013 KiCqStart® SYBR® Green Primers Gene Arrays Primer arrays for expression analysis of gene families
KCQS00 KiCqStart® SYBR® Green qPCR ReadyMix For Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems
KCQS01 KiCqStart® SYBR® Green qPCR ReadyMix Low ROX, for ABI and Stratagene instruments
KCQS02 KiCqStart® SYBR® Green qPCR ReadyMix with ROX for ABI instruments
KCQS03 KiCqStart® SYBR® Green qPCR ReadyMix iQ, with fluorescein for Bio-Rad systems
S4438 SYBR® Green JumpStart Taq ReadyMix for quantitative PCR, MgCI2 in buffer
S5193 SYBR® Green JumpStart Taq ReadyMix for quantitative PCR, without MgCl2 in buffer
S9194 SYBR® Green JumpStart Taq ReadyMix for High Throughput qPCR SYBR® Green qPCR reagent, passive reference dye included
S1816 SYBR® Green JumpStart Taq ReadyMix for Quantitative PCR, Capillary Formulation SYBR® Green qPCR reagent for Roche LightCycler® capillary systems