Probe Based Quantitative PCR

Probe based QPCR relies on the sequence-specific detection of a desired PCR product. Unlike SYBR® based QPCR methods that detect all double-stranded DNA, probe based QPCR utilizes a fluorescent-labeled target-specific probe resulting in increased specificity and sensitivity. Additionally, a variety of fluorescent dyes are available so that multiple primers can be used to simultaneously amplify many sequences.

PCR Technical Animations

• Ideal for high throughput. ReadyMixes contain all the necessary components for QPCR; you simply add the fluorescent detection chemistry, primers, and template.
• Specific. JumpStart Taq antibody prevents non-specific product formation through its hot-start mechanisms.
• Maximum flexibility in detection method since no detection method has been incorporated into the formulation.
• Optimized formulations with the addition of dUTP to facilitate carry–over prevention from previous PCR.

Product #

Description

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OLIGO Custom DNA and RNA Oligos Design and Order Custom DNA Oligos
PROBES Custom qPCR Probes Design and Order DNA, LNA, and BHQ Probes for qPCR
D6442 JumpStart Taq ReadyMix for High Throughput Quantitative PCR Ready-to-use 2x mix for qPCR with ROX
D7440 JumpStart Taq ReadyMix for Quantitative PCR For probe-based real-time PCR
L6669 LuminoCt® qPCR ReadyMix For fast probe-based quantitative PCR