FLAG® Antibodies

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Description

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Antibody Form

Immunogen

Application

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F7425 ANTI-FLAG® antibody produced in rabbit     affinity isolated antibody
DYKDDDDK
The antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays.

Browse additional application references in our FLAG® Literature portal.
SAB4200071 ANTI-FLAG® antibody, Rat monoclonal IgG1 6F7, monoclonal
purified immunoglobulin
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Monoclonal Anti-FLAG (rat IgG1 isotype) recognizes N-terminal, C-terminal and internal Flag-tagged fusion proteins. The product is especially recommended for identifying C-terminal FLAG-tagged fusion proteins.
F3040 Monoclonal ANTI-FLAG® M1 antibody produced in mouse IgG2b M1, monoclonal
purified immunoglobulin
DYKDDDDK
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunofluorescence (1 paper)
F1804 Monoclonal ANTI-FLAG® M2 antibody produced in mouse IgG1 M2, monoclonal
affinity isolated antibody
DYKDDDDK
For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation, immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.

Browse additional application references in our FLAG® Literature portal.
F3165 Monoclonal ANTI-FLAG® M2 antibody produced in mouse IgG1 M2, monoclonal
purified immunoglobulin
DYKDDDDK
Antibody is recommended for use in immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, ELISA, electron microscopy, flow cytometry and supershift assays.

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F4042 Monoclonal ANTI-FLAG® M5 antibody produced in mouse IgG1 M5, monoclonal
purified immunoglobulin
DYKDDDDK
Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.
Suitable for western blotting.

Browse additional application references in our FLAG® Literature portal.
F2555 Monoclonal ANTI-FLAG® antibody produced in rabbit IgG SIG1-25, monoclonal
ascites fluid
DYKDDDDK
Antibody suitable for use in immunoblotting,immunocytochemistry, and indirect ELISA.

Browse additional application references in our FLAG® Literature portal.