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Protease Inhibitor Cocktails for HIS-Select

For isolation of histidine-tagged proteins, Sigma-Aldrich offers several protease inhibitor cocktails without metal chelating agents like EDTA or 1,10-phenanthroline. The omission of metal chelators from these particular protease inhibitor cocktails ensures their compatibility with downstream immobilized metal affinity chromatography (IMAC) sample processing methods,1-3 while helping to preserve the recombinant proteins intact.

References
1. Bornhorst, J.A., and Falke, J.J., Purification of Proteins Using Polyhistidine Affinity Tags. Methods Enzymol., 326, 245-254 (2000).
2. Doyle, S.A., "Screening for the Expression of Soluble Recombinant Protein in Escherichia coli". Chemical Genomics: Reviews and Protocols, from Methods in Molecular Biology, Vol. 310 (ed. Edward D. Zanders), Humana Press (Totowa, NJ), ISBN 1-58829-399-8, pp. 115-122 (2005).
3. Murphy, M.B., and Doyle, S.A., "High-Throughput Purification of Hexahistidine-Tagged Proteins Expressed in E. coli". Chemical Genomics: Reviews and Protocols, from Methods in Molecular Biology, Vol. 310 (ed. Edward D. Zanders), Humana Press (Totowa, NJ), ISBN 1-58829-399-8, pp. 123-130 (2005).
4. Morigasaki, S., and Shiozahi, K., "Two-Component Signaling to the Stress NAO Kinase Cascade in Fission Yeast". Methods in Enzymology, 471, 279-290 (2010).

Product #

Description

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MSSAFE MS-SAFE Protease and Phosphatase Inhibitor
P8340 Protease Inhibitor Cocktail for use with mammalian cell and tissue extracts, DMSO solution
P8849 Protease Inhibitor Cocktail for use in purification of Histidine-tagged proteins, DMSO solution
P1860 Protease Inhibitor Cocktail for use in tissue culture media, DMSO solution
I3786 Protease Inhibitor Cocktail, Animal Component Free for use with mammalian cell and tissue extract, DMSO solution
S8830 SIGMAFAST Protease Inhibitor Cocktail Tablets, EDTA-Free for use in purification of Histidine-tagged proteins