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Trifluoromethanesulfonic acid

The GlycoProfile Chemical Deglycosylation Kit has been optimized to provide a rapid (~1 hour), convenient, and reproducible method to remove glycans from glycoproteins by reaction with trifluoromethanesulfonic acid (TFMS). The deglycosylated protein can then be recovered using a suitable downstream processing method. The kit contains sufficient reagents and a glycoprotein standard, for a minimum of 10 reactions when the sample size is between 1 and 2 mg of a typical glycoprotein. Unlike other chemical deglycosylation methods, hydrolysis with anhydrous TFMS is very effective at removing O and N-linked glycans (except the innermost Asn-linked GlcNAc or GalNAc).

Trifluoromethanesulfonic acid (TFMS) hydrolysis leaves an intact protein component, but results in destruction of the glycan. Glycoproteins from animals, plants, fungi, and bacteria have been deglycosylated by this procedure. Comparisons of functional interactions before and after treatment allow analysis of biological, immunological, and receptor binding properties. This permits a more complete understanding of the processing and roles in different stages of the glycoprotein′s lifecycle. The reaction is non-specific, removing all types of glycans, regardless of structure, although prolonged incubation is required for complete removal of O-linked glycans. Also, the innermost Asn-linked GlcNAc residue of N-linked glycans remains attached to the protein. The method removes the N-glycans of plant glycoproteins that are usually resistant to enzymatic hydrolysis.

Features and Benefits
  • Removes glycans from amino acids—permits sequence analysis of the protein by mass spectrometry, comparison DNA to protein sequence
  • Eliminates glycans affecting molecular radius—allows molecular mass determination by electrophoresis
  • Complete removal in single process—saves time by eliminating need for multiple enzyme reactions, overcoming enzyme resistance
  • Complete kit—eliminates waste and handling of excess reagents
For more information on Glycobiology please browse our online Glycobiology Analysis Manual.

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PP0510 GlycoProfile IV Chemical Deglycosylation Kit TFMS Deglycosylation System