Enzymatic Glycan Analysis

Terminal substitution of glycans with N-acetylneuraminic acid (NANA; sialic acid) has been associated with metabolic and cell signaling pathways. Sialylated glycoproteins are involved in cell-cell interactions associated with pathogenicity, signal recognition, immune response, tumor metastasis, and cell adhesion. Because of the terminal position of sialic acids and their acidic nature, sialylated glycoconjugates can act as inhibitors of intermolecular and intercellular reactions. Sialic acid content is often related to the efficacy of many glycoproteins of pharmaceutical interest and must be constantly monitored during the manufacturing process.

The Sialic Acid Quantitation Kit provides a rapid and accurate spectrophotometric method to quantitate sialic acid, either free, or in glycoproteins, cell surface glycoproteins, polysialic acids, or capsular polysaccharides (consisting only of polysialic acid). In addition, the kit can provide a simple and reliable method for the quantitation of sialic acid in sera and specific glycoconjugates to study disease states and virulence potentials in many pathogens. The Sialic Acid Quantitation Kit uses α(2→3,6,8,9) neuraminidase to cleave all sialic acid linkages, including α(2→8) and α(2→9) linkages, as well as branched sialic acid, for the most accurate determination of total sialic acid content.
For more information on Enzymatic Glycan Analysis please browse our online Glycobiology Analysis Manual.

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MAK314 Sialic Acid Assay Kit sufficient for colorimetric or fluorometric tests
SIALICQ Sialic Acid Quantitation Kit