Orchid Culture Media

We offer two proven media formulations for the tissue culture of the Orchid family. Of the approximately 25,000 Orchidaceae, many species are rare or endangered, others are prized horticulturally, and many are used in traditional medicine. Because orchids are difficult to grow from seed, Knudson C medium is used to support seed germination in tissue culture. For asexual orchid plant reproduction and multiplication from explants, Sigma’s Phytamax media serves well. Phytamax is half strength Murashige and Skoog medium, supplemented with sucrose, vitamins, peptone, and MES buffer. Maintainence Phytamax medium is available with or without charcoal and with both charcoal and banana powder. Phytamax for multiplication has the auxin NAA (naphtaleneacetic acid) included.

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K4003 Knudson C Modified Orchid Medium powder, plant cell culture tested
P6668 Phytamax Orchid Maintenance Medium powder, plant cell culture tested
P0931 Phytamax Orchid Maintenance Medium without Charcoal powder, plant cell culture tested
P1056 Phytamax Orchid Medium with Charcoal and Banana Powder powder, plant cell culture tested
P6793 Phytamax Orchid Multiplication Medium powder, plant cell culture tested