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Culture Media, Salts and Vitamin Mixes

The optimal tissue culture medium must be determined experimentally for each plant species. It is often necessary to change the medium formulation at different stages of culture, as when inducing callus to become plantlets. Likewise, the output of natural products or bioengineered molecules depends upon growing the cultured cells in properly formulated media. We offer the two most utilized basal media—Murashige - Skoog, and Gambourg’s B5—along with a choice of salt mixtures and vitamin mixtures. Each salt mixture contains different proportions of both the macronutrients nitrogen, potassium, calcium and magnesium as well as micronutrients such as iron and zinc. As an example, all salt mixtures contain cupric sulfate, but Hoagland’s has 3x compared to DKW/Juglans. A carbon source to support plant growth may be a sugar, or a more complex mixture such as coconut water, banana powder, or yeast, that we offer as well. For researchers’ convenience, Murashige and Skoog components are listed in several combinations.

Product #

Description

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B4032 Banana powder plant cell culture tested
C1416 Chu (N6) Basal Salt Mixture powder, plant cell culture tested
C5915 Coconut water suitable for plant cell culture
D6162 DKW/Juglans Basal Salt Mixture powder, plant cell culture tested
G5893 Gamborg’s B-5 Basal Medium with Minimal Organics fine powder, plant cell culture tested
G5768 Gamborg’s B-5 Basal Salt Mixture fine powder, plant cell culture tested
G1019 Gamborg’s Vitamin Solution 1000 ×, liquid, plant cell culture tested
H2395 Hoagland’s No. 2 Basal Salt Mixture powder, plant cell culture tested
M6774 McCown’s Woody Plant Basal Salt Mixture powder, plant cell culture tested
M6895 MEM Vitamin Solution (100×) sterile-filtered, BioReagent, suitable for cell culture
M0404 Murashige and Skoog Basal Medium powder, plant cell culture tested, with Gamborg′s vitamins
M5519 Murashige and Skoog Basal Medium powder, plant cell culture tested
M9274 Murashige and Skoog Basal Medium plant cell culture tested, with sucrose and agar
M5524 Murashige and Skoog Basal Salt Mixture (MS) powder, plant cell culture tested
M6899 Murashige and Skoog Basal Salts with minimal organics powder, plant cell culture tested
M7150 Murashige and Skoog Vitamin Powder 1000 ×, powder, plant cell culture tested
S6765 Schenk and Hildebrandt Basal Salt Mixture fine powder, plant cell culture tested
S3766 Schenk and Hildebrandt Vitamin 100 ×, liquid, plant cell culture tested
W0876 White’s Basal Salt Mixture powder, plant cell culture tested