Sequencing

The DNA sequence encodes everything about a plant, and knowing the specific nucleotide sequence provides not only identification, but information about breeding, signaling systems, growth, and more. Sequencing involves determining the order of nucleotides in a fragment of DNA or RNA. Common methods (protocols) include chain termination (also called dideoxy sequencing or the Sanger method, using sequence-specific termination of a DNA synthesis reaction by modified nucleotide substrates), pyrosequencing (enzymatically synthesizing the complement to a known single strand of DNA), with analysis by gel-based or capillary electrophoresis sequencing instruments. Clean-up columns remove interfering reaction components such as unincorporated dye-terminator nucleotides, salts and primers from sequencing reactions and radiolabeled nucleotides, primers, and fluorescent dyes from nucleic acid probe labeling reactions.

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B4930 Capillary Electrophoresis Running Buffer (10x) for automated DNA sequencing
S3938 SeqSaver Sequencing Premix Dilution Buffer for DNA sequencing
S5059 SigmaSpin Sequencing Reaction Clean-Up post-reaction clean-up columns
S4559 SigmaSpin Sequencing Reaction Clean-Up 50 post-reaction clean-up plates