Plasmid DNA Purification

Plasmids are small, circular units of non-genomic DNA that replicate within a cell independently of the chromosomal DNA. Plasmids are most commonly found in bacteria and are used in recombinant DNA research to transfer genes between cells. The Sigma catalog products use overnight, recombinant E. coli cultures as the source of plasmid DNA. Sigma’s GenElute High Performance Plasmid kits provide a rapid, non-hazardous method of isolating high quality plasmid DNA with silica-binding technology and spin column format. Recovered plasmid DNA is in super-coiled form, with no visual evidence of genomic DNA or RNA contamination, and ready for use in applications such as restriction digestion, ligation, sequencing, PCR, and transfection. The PhasePrep BAC DNA kit recovers large-molecular weight plasmids such as Bacterial Artificial Chromosomes in super-coiled form, free of RNA contamination, and with very low levels of endotoxin ready for immediate use in applications such as sequencing, restriction digestion, cloning, and PCR.

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NA0300S GenElute HP Plasmid Maxiprep Kit sufficient for 4 purifications Green Alternative
NA0300 GenElute HP Plasmid Maxiprep Kit sufficient for 10 purifications Green Alternative
NA0310 GenElute HP Plasmid Maxiprep Kit sufficient for 25 purifications Green Alternative
NA0200S GenElute HP Plasmid Midiprep Kit sufficient for 4 purifications Green Alternative
NA0200 GenElute HP Plasmid Midiprep Kit sufficient for 25 purifications Green Alternative
PLN10 GenElute Plasmid Miniprep Kit sufficient for 10 purifications Green Alternative
PLN70 GenElute Plasmid Miniprep Kit sufficient for 70 purifications Green Alternative
PLN350 GenElute Plasmid Miniprep Kit sufficient for 350 purifications Green Alternative
NA0100 PhasePrep BAC DNA Kit Scalable method for isolating large-molecular weight plasmids