Protein Identification

Proteins carry out the work in plant cells. One gene can code for multiple proteins; many proteins undergo post-translational modifications. Thus only direct identification of the total complement of proteins in an extract gives a true picture of the functioning units under different conditions or in different plant species. We offer proteomic grade endoproteinases for digestion of protein spots from 1- or 2-D gels. The resultant peptides can be sequenced by mass spectrometry, or in some cases by Edman degradation in a sequencer. In many cases tryptic peptides are sufficient for identifying a protein and its modifications. A particular protein may, for example, be phosphorylated in meristematic tissue, but not in roots. We also offer a set of kits to calibrate MALDI mass spectrometry instruments