Transformation of Yeast

Recombinant protein expression in Saccharomyces cerevisiae has many advantages such as simple protocols, use of basic reagents, and scalability. Explore the many products available for yeast transformation as part of the protein expression workflow.

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T2559 EZMix Tryptone Pancreatic digest of casein
L4158 Lithium acetate dihydrate BioXtra
N1127 2-Nitrophenyl β-D-galactopyranoside ≥98% (enzymatic)
84605 SD Agar for microbiology
T9285 Tris-EDTA buffer solution 100X concentrate for Northern and Southern blotting
GE28-9440-45 Yeast Buffer Kit GE Healthcare, 28-9440-45, pack of 1 ea
MITOISO3 Yeast Mitochondria Isolation Kit sufficient for 40 applications (using 20 OD culture preparations), isolation of an enriched mitochondrial fraction from yeast cells
Y1250 Yeast Nitrogen Base With Amino Acids Non-selective yeast growth medium
Y0626 Yeast Nitrogen Base Without Amino Acids Yeast classification medium
Y1751 Yeast Synthetic Drop-out Medium Supplements without histidine
Y2001 Yeast Synthetic Drop-out Medium Supplements without histidine, leucine, tryptophan and uracil
Y1376 Yeast Synthetic Drop-out Medium Supplements without leucine
Y0750 Yeast Synthetic Drop-out Medium Supplements without leucine and tryptophan
Y1876 Yeast Synthetic Drop-out Medium Supplements without tryptophan
Y1501 Yeast Synthetic Drop-out Medium Supplements without uracil
Y1771 Yeast Synthetic Drop-out Medium Supplements without uracil, leucine, and tryptophan
Y1896 Yeast Synthetic Drop-out Medium Supplements without lysine
Y2021 Yeast Synthetic Drop-out Medium Supplements without histidine, leucine, tryptophan, and adenine
Y2146 Yeast Synthetic Drop-out Medium Supplements without histidine, leucine, and tryptophan
YEAST1 Yeast Transformation Kit reagents for introducing plasmid DNA into yeast
Y1500 YPD Agar
Y1375 YPD Broth for microbiology