Agarose

Key properties associated with agaroses

Sulfate content: Used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength: The force that must be applied to a gel to cause it to fracture.
Gel point: The temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO): A movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Product #

Product Name

EEO

Transition Temp.

Gel Strength

Anion Traces

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A6236 Agarose - TAE Blend 1.0%          
A6361 Agarose - TAE Blend 2.0%          
A6111 Agarose - TBE Blend 1.0%          
AGRLE-RO Agarose LE low electroendosmosis          
AGRMP-RO Agarose MP multipurpose agarose          
AGRMS-RO Agarose MS molecular screening agarose          
A4018 Agarose, low gelling temperature   ≤0.10
congealing temperature <26-30 °C
≥200 g/cm2 (1% gel)
sulfate (SO42-): ≤0.10%
A9414 Agarose, low gelling temperature BioReagent, for molecular biology BioReagent ≤0.10
congealing temperature 26-30 °C
≥200 g/cm2 (1% gel)
sulfate (SO42-): ≤0.10%
A9045 Agarose, low gelling temperature BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture BioReagent, Cell Culture ≤0.1
congealing temperature 26-30 °C
≥200 g/cm2 (1% gel)
sulfate (SO42-): ≤0.10%
A0701 Agarose, low gelling temperature Type VII-A   ≤0.12
gel point 26 °C ±2 °C
≥250 g/cm2 (1% gel)
sulfate (SO42-): ≤0.4%
A7431 Agarose BioReagent, for molecular biology, Wide range/Standard 3:1   ≤0.13
transition temp 32.5-38 °C
≥1200 g/cm2 (4% gel)
sulfate (SO42-): ≤0.15%
A9539 Agarose BioReagent, for molecular biology, low EEO   0.09-0.13
gel point 36 °C (1.5% gel, ± 1.5 °C)
≥1200 g/cm2 (1% gel)
sulfate (SO42-): ≤0.15%
A2929 Agarose For pulsed field electrophoresis running gel   0.04-0.10
gel point 38-43 °C
≥2000 g/cm2
sulfate (SO42-): ≤0.20%
A3054 Agarose For pulsed field electrophoresis sample preparation   ≤0.10
gel point ≤30 °C
  sulfate (SO42-): ≤0.15%
A9793 Agarose High EEO   0.23-0.26
gel point 36 °C ±1.5 °C
>750 g/cm2 (1% gel)
sulfate (SO42-): <0.25%
05066 Agarose High EEO, for molecular biology Molecular Biology Reagent 0.23-0.27
gel point 34-37 °C (1.5% solution)
≥1500 g/cm2 (1.5% gel)
chloride (Cl-): ≤3000 mg/kg
sulfate (SO42-): ≤6000 mg/kg
A7174 Agarose High Gelling Temperature   ≤0.14
gel point 41 °C ±1.5 °C
≥1200 g/cm2 (1.5% gel)
≥900 g/cm2 (1% gel)
sulfate (SO42-): ≤0.20%
A4718 Agarose High resolution, for molecular biology Molecular Biology Reagent ≤.05
gel point ≤35 °C
≥300 g/cm2 (3% gel)
 
A0576 Agarose Low EEO   ≤0.12
gel point 36 °C ±1.5 °C (1.5% gel)
≥1800 g/cm2 (1% gel)
≥3200 g/cm2 (1.5% gel)
sulfate (SO42-): ≤0.12%
A4679 Agarose Low EEO, for Immunoelectrophoresis   0.09-0.13
gel point 34-38 °C
≥1200 g/cm2 (1% gel)
sulfate (SO42-): ≤0.20%
A6877 Agarose Medium EEO, for molecular biology Molecular Biology Reagent 0.16-0.19
congealing temperature 34.5-37.5 °C (1.5% gel)
≥1000 g/cm2 (1.0% gel)
sulfate (SO42-): ≤0.20%
A5304 Agarose Special High EEO Electrophoresis ≤0.40
gel point 36 °C ±1.5 °C
≥700 g/cm2 (1% gel)
sulfate (SO42-): ≤0.2%
A6013 Agarose Type I, low EEO   0.09-0.13
gel point 36 °C (1.5% gel, ±1.5 °C)
≥1200 g/cm2 (1% gel)
sulfate (SO42-): ≤0.15%
A0169 Agarose Type I-A, low EEO   0.09-0.13
gel point 36 °C ±1.5 °C
≥1200 g/cm2 (1% gel)
sulfate (SO42-): ≤0.20%
A9918 Agarose Type II-A, Medium EEO   0.16-0.19
gel point 36 °C ±1.5 °C
≥1000 g/cm2 (1% gel)
sulfate (SO42-): ≤0.25%
A3643 Agarose Type IV, Special High EEO   ≥0.3
     
A3893 Agarose Type VI, High Gelling Temperature   <0.1
gel point 42 °C ±1.5 °C
≥1000 g/cm2 (1% gel)
sulfate (SO42-): ≤0.20%
A5030 Agarose Ultra-low Gelling Temperature   ≤0.05
gel point 8-17 °C
   
A2576 Agarose Ultra-low Gelling Temperature, molecular biology grade Molecular Biology Reagent ≤0.12
gel point ≤20 °C
≥300 g/cm2 (1.5% gel)
sulfate (SO42-): ≤0.14%
A2790 Agarose Wide range, for molecular biology Molecular Biology Reagent ≤.15
gel point ≤35 °C
  sulfate (SO42-): ≤0.15%
E0264 Electrophoresis film for agarose gels sheet W × L 85 mm × 100 mm          
P5722 Precast Agarose Gels 20-well (landscape orientation)          
P5972 Precast Agarose Gels 24 wells (2 × 12-well portrait orientation)          
P6097 Precast Agarose Gels 24 wells (2 × 12-well portrait orientation)          
P5472 Precast Agarose Gels 8-well (portrait orientation)          
P6222 Precast Agarose Gels 8-well (portrait orientation)