Modifying Enzymes

During molecular cloning, it is often necessary to make changes to the nucleic acid to prepare it for ligation.  Sigma has a wide variety of enzymes to choose from.  For the most sensitive research, the Ultra Pure Modifying Enzymes offer the highest purity available.
 

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D7291 Deoxyribonuclease I RNase-free solution from bovine pancreas
D2886 DNA Ligase from T4-infected Escherichia coli buffered aqueous glycerol solution
LIG1 DNA Ligation Kit
DNAPOL-RO DNA Polymerase I from Escherichia coli lysogenic for NM 964
D9380 DNA Polymerase I from Escherichia coli lysogenic for NM 964 buffered aqueous glycerol solution
D8276 DNA Polymerase I, Klenow Fragment from Escherichia coli buffered aqueous glycerol solution
E1131 Exonuclease III from Escherichia coli BE25 /psGR3 buffered aqueous glycerol solution
G5663 Glutathione S-Transferase from E. coli recombinant, expressed in E. coli, buffered aqueous solution
P4978 Phosphatase, Alkaline from calf intestine buffered aqueous glycerol solution
P4252 Phosphatase, Alkaline from Escherichia coli ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)
P4069 Phosphatase, Alkaline from Escherichia coli buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)
PNK-RO Polynucleotide Kinase from Escherichia coli E. D. pFDX
P4390 Polynucleotide Kinase from T4-infected Escherichia coli 10 units/μL, buffered aqueous glycerol solution
P6911 Protease from Streptomyces griseus BioReagent, DNase, RNase, and nickase, none detected (No RNase.)
P2308 Proteinase K from Tritirachium album lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology
P4850 Proteinase K from Tritirachium album buffered aqueous glycerol solution, for molecular biology, ≥800 units/mL
P9290 Proteinase K–Agarose from Tritirachium album lyophilized powder
LIG2 QuickLink DNA Ligation Kit joins blunt-end and sticky-end DNA fragments
R6513 Ribonuclease A from bovine pancreas for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
R4642 Ribonuclease A from bovine pancreas (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
RPOLSP6-RO SP6 RNA Polymerase from Escherichia coli BL 21/pSR3
RPOLT3-RO T3 RNA Polymerase from Escherichia coli HB101
T4DNAPOL-RO T4 DNA Polymerase from T4 plasmid pTL43W infected Escherichia coli 71–18
T4PNK-RO T4 Polynucleotide Kinase, 3′-phosphatase free from phage T4 am N81 pse T1 infected Escherichia coli BB
R0884 T7 RNA Polymerase recombinant, expressed in E. coli, buffered aqueous solution
RPOLT7-RO T7 RNA Polymerase from Escherichia coli BL 21/pAR 1219
RTT-RO Terminal Transferase from Calf Thymus, recombinant, E. coli
T4427 Terminal Transferase from calf thymus buffered aqueous glycerol solution
T7428 Topoisomerase I from wheat germ buffered aqueous glycerol solution