| Acc��I from Acinetobacter calcoaceticus buffered aqueous glycerol solution | Recognition Sequence: 5'-GT/(A,C)(G,T)AC-3'
Ligation and recutting results: After 2-10-fold Acc��I overdigestion of 1��μg pBR322 DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >90% recut.
Heat inactivation: Activity retained after 10 minutes at 65��°C. Five-fold enhanced activity occurs at 55°C. Enzyme inactivated at 80��°C for 20 minutes.
| R6142 | pricing |
|
| Alu��I from Arthrobacter luteus buffered aqueous glycerol solution | Recognition Sequence: 5'-AG/CT-3'
Ligation and recutting results: After 2-10-fold Alu��I overdigestion of 1��μg λ DNA substrate, results in 95% cutting, >80% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65��°C for 15 minutes.
| R6885 | pricing |
|
| Apa��I from Acetobacter pasteurianus buffered aqueous glycerol solution | Recognition Sequence: 5'-GGGCC/C-3'
Ligation and recutting results: After 2-10-fold Apa��I overdigestion of Ad-2 DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65��°C for 15 minutes.
| R4258 | pricing |
|
| Ava��II from Anabaena variabilis buffered aqueous glycerol solution | Recognition sequence: 5′-G/G(A,T)CC-3′
Ligation and recutting results: After 2-10-fold Ava��II overdigestion of 1��μg λ DNA substrate, results in 100%cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: 65��°C for 15 minutes.
| R6004 | pricing |
|
| BamH ��I from Bacillus amyloliquefaciens H buffered aqueous glycerol solution | Recognition sequence: 5′-G/GATCC-3′
Ligation and recutting results: After 2-10-fold Bam��HI overdigestion of 1��μg λ DNA substrate, results in 100% cutting, 95% of fragments can be ligated, and 95%recut.
Heat inactivation: 60��°C for 15 minutes.
Star activity: To prevent star activity, avoid suboptimal reaction conditions containing low salt concentration, high glycerol (>5%) and high pH 8.0.
| R0260 | pricing |
|
| Bcl��I from Bacillus caldolyticus buffered aqueous glycerol solution | Recognition sequence: 5′-T/GATCA-3′
Ligation and recutting results: After 2-10-fold Bcl��I overdigestion of 1��μg λ DNA substrate, results in 100% cutting, 95% of fragments can be ligated, and 95% recut.
Heat inactivation: This enzyme cannot be heat inactivated at 65��°C for 15 minutes.
| R8631 | pricing |
|
| Bgl II from Bacillus licheniformis buffered aqueous glycerol solution | Recognition sequence: 5′-A/GATCT-3′
Ligation and recutting results: After 2-10-fold Bgl��II overdigestion of 1��μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and >95% recut.
Heat Inactivation: This enzyme cannot be heat inactivated.
| R6377 | pricing |
|
| Bgl��I from Bacillus licheniformis buffered aqueous glycerol solution | Recognition sequence: 5′-GCC(N)4/NGGC-3′
Ligation and recutting results: After 2-10-fold Bgl��I overdigestion of 1��μg λ DNA substrate, results in 100% cutting, >70% of fragments can be ligated, and >90% recut.
Heat inactivation: Inactivation is achieved at 65��°C for 15 minutes.
| R6753 | pricing |
|
| Bln��I from Brevibacterium linens buffered aqueous glycerol solution | Recognition sequence: 5′-C/CTAGG-3′
Ligation and recutting results: After 2-10-fold Bln��I overdigestion of 1��μg λ DNA substrate, results in 100% cutting, >70% of fragments can be ligated, and >90% recut.
Heat inactivation:. This enzyme is not heat inactivated at 65��°C for 15 minutes.
| R3131 | pricing |
|
| Bsm��I from Bacillus stearothermophilus NUB��36 buffered aqueous glycerol solution | Recognition sequences: 5'-GAATGCN/-3' 3'-CTTAC/GN 5
Ligation and recruiting results: After 2-10-fold Bsm��I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: Heat inactivation for Bsm I is not available.
| R3635 | pricing |
|
| BssH��II from Bacillus stearothermophilus buffered aqueous glycerol solution | Recognition sequence: 5′-G/CGCGC-3′
Ligation and recutting results: After 2-10-fold BssH��II overdigestion of 1��μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Sensitive and inactivated at 80��°C for 20��minutes.
Comment: Optimal activity is at 50��°C
| R2634 | pricing |
|
| BstE��II from Bacillus stearothermophilus ET buffered aqueous glycerol solution | Recognition sequence: 5′-G/GTNACC-3′
Ligation and recutting results: After 2-10-fold BstE��II overdigestion of 1��μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: This enzyme cannot be inactivated at 65��°C for 15��minutes.
| R4253 | pricing |
|
| Cfo��I from Clostridium formicoaceticum buffered aqueous glycerol solution | Recognition Sequence: 5′-GCG/C-3′
Ligation and recutting results: After 2-10-fold Cfo��I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Not complete at 65��°C for 10 minutes.
| R1761 | pricing |
|
| Cla��I from Caryophanon latum��L buffered aqueous glycerol solution | Recognition sequence: 5′-AT/CGAT-3′
Ligation and recutting results: After 2-10-fold Cla��I overdigestion of 1��μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: Enzyme is not inactivated at 65��°C for 20��minutes.
| R7763 | pricing |
|
| Dde��I from Desulfovibrio desulfuricans strain Norway buffered aqueous glycerol solution | Recognition sequence: 5′-C/TNAG-3′
Ligation and recutting results: After 2-10-fold Dde��I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Completed after 65��°C incubation for 20��minutes.
Comment: Single-stranded DNA cleaves slowly.
| R4256 | pricing |
|
| Dpn��I from Diplococcus pneumoniae buffered aqueous glycerol solution | Recognition sequence: 5′-GmA/TC-3′
Ligation and recutting results: After 2-10-fold Dpn��I overdigestion of 1 μg pBR322 DNA substrate, results in 100% cutting, >30% of fragments can be ligated, and >90% recut.
Heat inactivation: 75��°C for 15 minutes.
| R8381 | pricing |
|
| Dra��I from Deinococcus radiophilus buffered aqueous glycerol solution | Recognition sequence: 5′-TTT/AAA-3′
Ligation and recutting results: After 2-10-fold Dra��I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >70% of fragments can be ligated, and >90% recut.
Heat inactivation: 65��°C for 20 minutes.
| R4381 | pricing |
|
| EclX��I from Enterobacter cloacae 590 buffered aqueous glycerol solution | Recognition sequence: 5′-C/GGCCG-3′
Ligation and recutting results: After 2-10-fold EclX��I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65��°C for 20 minutes.
| R3884 | pricing |
|
| EcoR��I from Escherichia coli BS5 buffered aqueous glycerol solution | Recognition sequence: 5′-G/AATTC-3′
Ligation and recutting results: After 2-10-fold EcoR��I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, 95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65��°C for 20 minutes.
| R6265 | pricing |
|
| EcoR��I from Escherichia coli BS5 buffered aqueous glycerol solution | Recognition sequence: 5′-G/AATTC-3′
Ligation and recutting results: After 2-10-fold EcoR��I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, 95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65��°C for 20 minutes.
| R4640 | pricing |
|
| EcoR��II from Escherichia coli MV1 193 buffered aqueous glycerol solution | Recognition sequence:5′-/CC(AT)GG-3′
Ligation and recutting results: After 2-10-fold EcoR II overdigestion of 1 μg Ad-2 DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >90% recut.
Heat inactivation: 65 °C for 10 minutes.
| R1636 | pricing |
|
| EcoR��V from Escherichia coli buffered aqueous glycerol solution | Recognition sequence: 5′-GAT/ATC-3′
Ligation and recutting results: After 2-10-fold Eco��RV overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >85% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.
| R2756 | pricing |
|
| Hae��III from Haemophilus aegyptius buffered aqueous glycerol solution | Recognition sequence: 5′-GG/CC-3′
Ligation and recutting results: After 2-10-fold Hae��III overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >50% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.
| R5628 | pricing |
|
| Hind��II from Haemophilus influenzae Rd buffered aqueous glycerol solution | Recognition sequence: 5′-GTPy/PuAC-3′
Ligation and recutting results: After 2-10-fold Hind II overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >70% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 15 minutes.
| R1382 | pricing |
|
| Hind��III from Haemophilus influenzae buffered aqueous glycerol solution | Recognition sequence: 5′-A/AGCTT-3′
Ligation and recutting results: After 2-10-fold Hind III overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 15 minutes.
| R1137 | pricing |
|
| Hpa��I from Haemophilus parainfluenzae buffered aqueous glycerol solution | Recognition sequence: 5′-GTT/AAC-3′
Ligation and recutting results: After 2-10-fold Hpa��I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and >95% recut.
Heat inactivation: Not completely inactivated at 65��°C for 15 minutes.
| R8507 | pricing |
|
| Hpa��II from Haemophilus parainfluenzae buffered aqueous glycerol solution | Recognition sequence: 5′-C/CGG-3′
Ligation and recutting results: After 2-10-fold Hpa��II overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65��°C for 20 minutes.
| R0629 | pricing |
|
| Kpn��I from Klebsiella pneumoniae buffered aqueous glycerol solution | Recognition sequence: 5′-GGTAC/C-3′
Ligation and recutting results: After 2-10-fold Kpn��I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65��°C for 15 minutes.
| R1258 | pricing |
|
| Ksp��I from Kluyvera sp. buffered aqueous glycerol solution | Recognition sequence: 5′-CCGC/GG-3′
Ligation and recutting results: After 2-10-fold Ksp��I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >90% recut.
| R4134 | pricing |
|
| MluN��I from Micrococcus luteus strain N buffered aqueous glycerol solution | Recognition sequence: 5′-TGG/CCA-3′
Ligation and recutting results: After 2-10-fold MluN��I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >85% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 10 minutes.
| R1507 | pricing |
|
| Mlu��I from Micrococcus luteus (lysodeikticus) buffered aqueous glycerol solution | Recognition sequence: 5′-A/CGCGT-3′
Ligation and recutting results: After 2-10-fold Mlu��I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Partially inactivated at 65��°C for 10��minutes.
| R8257 | pricing |
|
| Msp��I from Moraxella sp. buffered aqueous glycerol solution | Recognition sequence: 5′-C/CGG-3′
Ligation and recutting results: After 2-10-fold Msp I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65��°C for 15 minutes.
| R4506 | pricing |
|
| Mva��I from Micrococcus varians Rfl��19 buffered aqueous glycerol solution | Recognition sequence: 5′-CC/(A,T)GG-3′
Ligation and recutting results: After 2-10-fold Mva I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >90% recut
Heat inactivation: Inactivated at 60 °C for 15 minutes.
| R1632 | pricing |
|
| Nco��I from Nocardia corallina buffered aqueous glycerol solution | Recognition sequence: 5′-C/CATGG-3′
Ligation and recutting results: After 2-10-fold Nco��I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65��°C for 15 minutes.
| R8761 | pricing |
|
| Nde��I from Neisseria denitrificans buffered aqueous glycerol solution | Recognition sequence: 5′-CA/TATG-3′
Ligation and recutting results: After 2-10-fold Nde��I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 60��°C for 15 minutes.
| R5509 | pricing |
|
| Nhe��I from Neisseria mucosa heidelbergensis buffered aqueous glycerol solution | Recognition sequence: 5′-G/CTAGC-3′
Ligation and recutting results: After 2-10-fold Nhe��I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >80% recut.
Heat inactivation: Inactivated at 65��°C for 20 minutes.
| R5634 | pricing |
|
| Not��I from Nocardia otidiscaviarum buffered aqueous glycerol solution | Recognition sequence: 5′-GC/GGCCGC-3′
Ligation and recutting results: After 2-10-fold Not��I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and 95% recut.
Heat inactivation: Inactivated at 65��°C for 15 minutes.
| R8506 | pricing |
|
| Nsi��I from Neisseria sicca buffered aqueous glycerol solution | Recognition sequence: 5′-ATGCA/T-3′
Ligation and recutting results: After 2-10-fold Nsi I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >90% recut.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
| R5884 | pricing |
|
| Pst��I from Providencia stuartii buffered aqueous glycerol solution | Recognition sequence: 5′-CTGCA/G-3′
Ligation and recutting results: After 2-10-fold Pst I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 80 °C for 20 minutes.
| R7023 | pricing |
|
| Pvu��I from Proteus vulgaris buffered aqueous glycerol solution | Recognition sequence: 5′-CGAT/CG-3′
Ligation and recutting results: After 2-10-fold Pvu��I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 80��°C for 20 minutes.
| R1508 | pricing |
|
| Pvu��II from Proteus vulgaris buffered aqueous glycerol solution | Recognition sequence: 5′-CAG/CTG-3′<
Ligation and recutting results: After 2-10-fold Pvu��II overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and >90% recut.
Heat inactivation: Activity not completely destroyed at 65��°C for 15 minutes.
| R2631 | pricing |
|
| Rsa��I from Rhodopseudomonas sphaeroides buffered aqueous glycerol solution | Recognition sequence: 5′-GT/AC-3′
Ligation and recutting results: After 2-10-fold Rsa��I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >50% of fragments can be ligated, and 95% recut.
Heat inactivation: Inactivated at 65��°C for 20 minutes.
| R4756 | pricing |
|
| SAC™��I from Streptomyces achromogenes buffered aqueous glycerol solution | Recognition sequence: 5′-GAGCT/C-3′
Ligation and recutting results: After 2-10-fold Sac��I overdigestion of 1 μg λ Hind III DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation : Inactivated at 65��°C for 15 minutes.
| R5268 | pricing |
|
| Sal��I from Streptomyces albus G buffered aqueous glycerol solution | Recognition sequence: 5′-G/TCGAC-3′
Ligation and recutting results: After 2-10-fold Sal I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 15 minutes.
| R0754 | pricing |
|
| Sau3A I from Staphylococcus aureus buffered aqueous glycerol solution | Recognition sequence: 5′-/GATC-3′
Ligation and recutting results: After 2-10-fold Sau3A I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 20 minutes.
| R0762 | pricing |
|
| Sca��I from Streptomyces caespitosus buffered aqueous glycerol solution | Recognition sequence: 5′-AGT/ACT-3′
Ligation and recutting results: After 2-10-fold Sca��I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >90% recut.
Heat inactivation: Inactivated at 80��°C for 20 minutes.
| R5007 | pricing |
|
| Sfi��I from Streptomyces fimbriatus buffered aqueous glycerol solution | Recognition sequence: 5′-GGCC(N)4/NGGCC-3′
Ligation and recutting results: After 2-10-fold Sfi I overdigestion of 1 μg Ad-2 DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and >95% recut.
| R8256 | pricing |
|
| Sma��I from Serratia marcescens Sb buffered aqueous glycerol solution | Recognition sequence: 5′-CCC/GGG-3′
Ligation and recutting results: After 2-10-fold Sma I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and >80% recut.
Heat inactivation: 65 °C for 15 minutes.
| R4503 | pricing |
|
| Spe��I from Sphaerotilus sp. buffered aqueous glycerol solution | Recognition sequence: 5′-A/CTAGT-3′
Ligation and recutting results: After 2-10-fold Spe I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
| R5257 | pricing |
|
| Sph��I from Streptomyces phaeochromogenes buffered aqueous glycerol solution | Recognition sequence: 5′-GCATG/C-3′
Ligation and recutting results: After 2-10-fold Sph��I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65��°C for 20 minutes.
| R7135 | pricing |
|
| Ssp��I from Sphaerotilus sp. buffered aqueous glycerol solution | Recognition sequence: 5′-AAT/ATT-3′
Ligation and recutting results: After 2-10-fold Ssp��I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65��°C for 20 minutes.
| R6759 | pricing |
|
| Sty��I from Escherichia coli strain carrying pST27 buffered aqueous glycerol solution | Recognition sequence: 5′-C/C(A,T)(T,A)GG-3′
Ligation and recutting results: After 2-10-fold Sty I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 15 minutes.
| R7009 | pricing |
|
| Taq I from Thermus aquaticus recombinant, expressed in Escherichia coli (Strain that carries a Taq I overproducing plasmid.), buffered aqueous glycerol solution | Recognition sequence: 5′-T/CGA-3′
Ligation and recutting results: After 2-10-fold Taq��I overdigestion of 1 μg λ DNA substrate, results in >95% cutting, >85% of fragments can be ligated, and >95% recut.
Heat inactivation: 80��°C for 20 minutes.
| R9507 | pricing |
|
| Xba��I from Xanthomonas badrii buffered aqueous glycerol solution | Recognition sequence: 5′-T/CTAGA-3′
Ligation and recutting results: After 2-10-fold Xba��I overdigestion of 1 μg Ad-2 DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: Up to 15 units of enzyme inactivated at 65��°C for 15 minutes.
| R7260 | pricing |
|
| Xho��I from Xanthomonas holcicola buffered aqueous glycerol solution | Recognition sequence: 5′-C/TCGAG-3′
Ligation and recutting results: After 2-10-fold Xho��I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65��°C for 15 minutes.
| R6379 | pricing |
|
Restriction Enzyme 