PCR Reaction Components

Reduce the risk of contamination and faulty PCR results with Sigma’s certified PCR graded reagents. Where applicable, PCR graded reagents are assayed to ensure no risk of DNase, RNase contamination and also validated for use in PCR.
Enhance your PCR results today with Sigma’s PCR graded reagents.
  • Validated Performance – Sigma’s PCR graded reagents are fully validated to ensure suitability in PCR.
  • Ultrapure Tested – Minimize the risk of contamination with Ultrapure PCR grade reagents certified both DNase & RNase free.

Product #

Description

Application

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B0300 Betaine solution 5 M, PCR Reagent The addition of betaine at a final concentration of 0.8-1.6M improves the amplification of DNA by reducing the formation of secondary structure in GC-rich regions. The addition of betaine has been reported to enhance the specificity of the polymerase chain reaction by eliminating the base pair composition dependence of DNA melting.
53833 BioStab PCR optimizer (II)  
D9170 Dimethyl sulfoxide PCR Reagent Dimethyl sulfoxide (1-10%) has been shown to accelerate strand renaturation and is believed to give the nucleic acid thermal stability against depurination. As a PCR cosolvent, DMSO may help improve yields, especially in long PCR.
M8787 Magnesium chloride solution PCR Reagent, 25 mM MgCI2 solution for PCR • For routine PCR amplifications.
• Optimization of MgCl2 concentration in PCR reactions for improved yield.

11699113001 MgCl2 Stock Solution The combination of PCR Buffer without MgCl2 and the MgCl2 Stock Solution buffer are optimized for the amplification of specific DNA fragments in conventional PCRs (i.e., with Taq DNA Polymerase). However, the MgCl2 Stock Solution may be used with other, more appropriate PCR buffers to optimize any type of PCR (e.g., hot start PCR, high fidelity PCR, long template PCR).
M8662 Mineral oil PCR Reagent • For routine PCR amplifications.
• Minimizes evaporation of reactions run in PCR instruments without heated lids.

P2192 10X PCR Buffer Optimized for routine PCR with MgCl2 included For routine PCR amplifications
11699105001 PCR Buffer Without MgCl2, 10x concentrated The PCR buffer without MgCl2 is used for the amplification of specific DNA fragments by the polymerase chain reaction (PCR).
The buffer is ten-fold concentrated and should be used in combination with the MgCl2 Stock Solution.
11034731001 Primer "random" Primer for cDNA synthesis
R1158 Ribonuclease Inhibitor recombinant, expressed in E. coli, 20-40 units/μL Suitable for use in
• in vitro inhibition of ribonucleases
• cDNA synthesis
• RT-PCR
• in vitro transcription and translation

Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.
R2520 Ribonuclease inhibitor human Inhibits ribonucleases during transcription and translation experiments Suitable for use in
• in vitro inhibition of ribonucleases
• cDNA synthesis
• RT-PCR
• in vitro transcription and translation

Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.
S3917 Single-strand Binding Protein from Escherichia coli >95% (SDS-PAGE), for molecular biology, Single strand binding protein to enhance PCR specificity Binds with high specificity to single-stranded DNA. Can be useful in enhancing the specificity of PCR and in enabling the sequencing of problematic DNA templates.
T4GENE32-RO T4 Gene 32 Protein T4 Gene 32 Protein is a single-strand-specific DNA-binding protein. It may be used for:
• Optimization of PCR (See "Product Description" below.)
• Stimulation of in vitro DNA synthesis
• Stabilization of single-stranded regions of DNA or RNA
• Site-specific mutagenesis experiments (with T4 DNA polymerase and T4 DNA ligase)
• Helping restriction enzyme digestions go to completion
• q-PCR

W1754 Water PCR Reagent  
PCRH20-RO Water, PCR Grade This product is specially tested and qualified for use in all PCR or RT-PCR. It can be used in conjunction with all end-point or real-time PCR assays or whenever highest quality water is needed. All of the LightCycler® kits and some of the amplification products — available from Roche Applied Science — contain this Water, PCR Grade.