PCR Reaction Components

Reduce the risk of contamination and faulty PCR results with Sigma’s certified PCR graded reagents. Where applicable, PCR graded reagents are assayed to ensure no risk of DNase, RNase contamination and also validated for use in PCR.

Enhance your PCR results today with Sigma’s PCR graded reagents.

• Validated Performance – Sigma’s PCR graded reagents are fully validated to ensure suitability in PCR.

• Ultrapure Tested – Minimize the risk of contamination with Ultrapure PCR grade reagents certified both DNase & RNase free.

Product #

Description

Application

Add to Cart
B0300 Betaine solution 5 M, PCR Reagent The addition of betaine at a final concentration of 0.8-1.6M improves the amplification of DNA by reducing the formation of secondary structure in GC-rich regions. The addition of betaine has been reported to enhance the specificity of the polymerase chain reaction by eliminating the base pair composition dependence of DNA melting.1
    • 53833 BioStab PCR optimizer (II)  
    • D9170 Dimethyl sulfoxide PCR Reagent Dimethyl sulfoxide (1-10%) has been shown to accelerate strand renaturation and is believed to give the nucleic acid thermal stability against depurination. As a PCR cosolvent, DMSO may help improve yields, especially in long PCR.
    • M8787 Magnesium chloride solution PCR Reagent, 25 mM MgCI2 solution for PCR  
    • M8662 Mineral oil PCR Reagent  
    • P2192 10X PCR Buffer Optimized for routine PCR with MgCl2 included  
    • PCRII PCR Buffer without MgCl2 10 × The PCR Buffer, diluted to 1×, provides the recommended pH and ionic strength. The MgCl2 can be used to optimize the Mg2+ concentration for PCR with any template/primer set.
    • R1158 Ribonuclease Inhibitor recombinant, expressed in E. coli, 20-40 units/μL Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.
    • R2520 Ribonuclease inhibitor human Inhibits ribonucleases during transcription and translation experiments Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.
    • S3917 Single-strand Binding Protein from Escherichia coli >95% (SDS-PAGE), for molecular biology, Single strand binding protein to enhance PCR specificity Binds with high specificity to single-stranded DNA. Can be useful in enhancing the specificity of PCR and in enabling the sequencing of problematic DNA templates.
    • U6760 Uracil-DNA glycosylase from Escherichia coli ≥90% (SDS-PAGE), recombinant, expressed in E. coli, 3,000 units/mL, buffered aqueous solution Uracil-DNA glycosylase has been used to degrade amplicon DNA generated with dUTP to help reduce the rate of false positives in RT-PCR analysis. 1
    • W1754 Water PCR Reagent