RT-PCR and RT-qPCR

RT-PCR combines two powerful and versatile techniques, reverse transcription and the polymerase chain reaction to generate and amplify cDNA from total RNA or mRNA transcripts. The method is often used to study gene expression at both the RNA and protein levels.The ideal RT-PCR requires a sensitive reverse transcription and a high fidelity amplification. The reverse transcriptase should be able to detect very low abundance transcripts and/or transcripts containing difficult secondary structure. Sigma’s enhanced Avian (eAMV™) Reverse Transcriptase displays all of these characteristics. It is the ideal RT for detecting low abundance messages that may be missed by other reverse transcriptases and it is the best enzyme we have found for transcribing through difficult secondary structure. It is also more tolerant to elevated temperatures than standard AMV, M-MLV, M-MLV RNase H– or AMV RNase H reduced.
An Overview of Reverse Transcription PCR (RT-PCR)

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OLIGO Custom DNA and RNA Oligos Design and Order Custom DNA Oligos
PROBES Custom qPCR Probes Design and Order DNA, LNA, and BHQ Probes for qPCR
AMPD1 DNase I Amplification Grade
STR1 Enhanced Avian First Strand Synthesis Kit Components for cDNA synthesis with enhanced AMV reverse transcriptase
HSRT100 Enhanced Avian HS RT-PCR Kit Flexible kit for one-step or two-step RT-PCR
A4464 Enhanced Avian Reverse Transcriptase [eAMV RT] For reverse transcription at higher temperatures & rare mRNAs
ERT-RO Expand Reverse Transcriptase
GE27-9261-01 First-strand cDNA Synthesis Kit GE Healthcare, 27-9261-01
11483188001 First Strand cDNA Synthesis Kit for RT-PCR (AMV)
KCQS07 KiCqStart® One-Step Probe RT-qPCR ReadyMix for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems
KCQS08 KiCqStart® One-Step Probe RT-qPCR ReadyMix, Low ROX with Low ROX for ABI and Stratagene instruments
KCQS09 KiCqStart® One-Step Probe RT-qPCR ReadyMix, ROX with ROX for ABI instruments
KCQS04 KiCqStart® Probe qPCR ReadyMix for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems
KCQS05 KiCqStart® Probe qPCR ReadyMix, Low ROX with Low ROX for ABI and Stratagene instruments
KCQS06 KiCqStart® Probe qPCR ReadyMix, ROX with ROX for ABI instruments
KSPQ12012 KiCqStart® SYBR® Green Primers Predesigned primers for gene expression analysis
KSGA2013 KiCqStart® SYBR® Green Primers Gene Arrays Primer arrays for expression analysis of gene families
M1302 M-MLV Reverse Transcriptase Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis
O4387 Oligo(dT)23, Anchored 70 μM in H2O
10814270001 Primer for cDNA Synthesis
RNAINH-RO Protector RNase Inhibitor
QR0200 Quantitative RT-PCR ReadyMix One step RT-qPCR for probe-based methods, MMLV & hot-start Taq
R7647 Random Nonamers for use as primers in cDNA synthesis, 50 μM in H2O
RDRT ReadyScript® cDNA Synthesis Mix Complete reagent for first strand cDNA synthesis for RT-qPCR
GE27-9263-01 Ready-to-go T-Primed First-Strand Kit GE Healthcare, 27-9263-01, sufficient for 55 reactions
10109118001 Reverse Transcriptase AMV
11062603001 Reverse Transcriptase M-MuLV
R2520 Ribonuclease inhibitor human Inhibits ribonucleases during transcription and translation experiments
10786357001 Ribonuclease H (RNase H) from Escherichia coli H 560 pol A1
QR0100 SYBR® Green Quantitative RT-qPCR Kit One step SYBR® Green RT-qPCR with MMLV & hot-start Taq DNA Polymerase
11939823001 Titan One Tube RT-PCR Kit
11855476001 Titan One Tube RT-PCR System
11480022001 Tth DNA Polymerase