RT-PCR

RT-PCR combines two powerful and versatile techniques, reverse transcription and the polymerase chain reaction to generate and amplify cDNA from total RNA or mRNA transcripts. The method is often used to study gene expression at both the RNA and protein levels.The ideal RT-PCR requires a sensitive reverse transcription and a high fidelity amplification. The reverse transcriptase should be able to detect very low abundance transcripts and/or transcripts containing difficult secondary structure. Sigma’s enhanced Avian (eAMV™) Reverse Transcriptase displays all of these characteristics. It is the ideal RT for detecting low abundance messages that may be missed by other reverse transcriptases and it is the best enzyme we have found for transcribing through difficult secondary structure. It is also more tolerant to elevated temperatures than standard AMV, M-MLV, M-MLV RNase H– or AMV RNase H reduced.
An Overview of Reverse Transcription PCR (RT-PCR)

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R9376 AMV-Reverse Transcriptase from avian myeloblastosis virus
AMPD1 DNase I Amplification Grade
STR1 Enhanced Avian First Strand Synthesis Kit Components for cDNA synthesis with enhanced AMV reverse transcriptase
HSRT100 Enhanced Avian HS RT-PCR Kit Flexible kit for one-step or two-step RT-PCR
A4464 Enhanced Avian Reverse Transcriptase [eAMV RT] For reverse transcription at higher temperatures & rare mRNAs
M1302 M-MLV Reverse Transcriptase Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis
O4387 Oligo(dT)23, Anchored 70 μM in H2O
QR0200 Quantitative RT-PCR ReadyMix One step RT-qPCR for probe-based methods, MMLV & hot-start Taq
QR0100 SYBR® Green Quantitative RT-qPCR Kit One step SYBR® Green RT-qPCR with MMLV & hot-start Taq DNA Polymerase