Routine PCR Amplification

PCR Enzymes and Kits

What is Taq DNA polymerase?

Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus which lives in hot springs and hydrothermal vents. This enzyme is able to withstand repeated heating to 95 °C, which is necessary for PCR, without significant loss of activity. This eliminates the need to replace the enzyme after each amplification cycle. Taq polymerase has a molecular weight of 94 kDa and shows 5′→ 3′ exonuclease activity. The enzyme is homologous to, and replaces the previously used DNA polymerase I, Klenow fragment, derived from E. coli.

References
1. Gelfand, David H. "Taq DNA polymerase." PCR technology. Palgrave Macmillan UK, 1989. 17-22.
2. Eom, Soo Hyun, Jimin Wang, and Thomas A. Steitz. "Structure of Taq polymerase with DNA at the polymerase active site." Nature 382.6588 (1996): 278-281.
3. Holland, Pamela M., et al. "Detection of specific polymerase chain reaction product by utilizing the 5′→ 3′ exonuclease activity of Thermus aquaticus DNA polymerase." Proceedings of the National Academy of Sciences 88.16 (1991): 7276-7280.
4. Dahlberg, Michael E., and Stephen J. Benkovic. "Kinetic mechanism of DNA polymerase I (Klenow fragment): identification of a second conformational change and evaluation of the internal equilibrium constant." Biochemistry 30.20 (1991): 4835-4843.

Standard Taq is offered in multiple formats:
  • Taq and Buffer (with Magnesium Chloride in buffer or separate)
  • ReadyMixes (Taq, buffer, ions and dNTPs in an optimized master mix)

We also offer Red versions of standard Taq that contain an inert dye that acts as a visual tracker and built in loading dye.

Product #

Description

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APTAT-RO AptaTaq Fast DNA Polymerase
ATPAM-RO AptaTaq Fast PCR Master
OLIGO Custom DNA and RNA Oligos Design and Order Custom DNA Oligos
11699121001 PCR Buffer Set
11578553001 PCR Core Kit
11585541001 PCR Core KitPLUS
CORET PCR Core kit with Taq DNA polymerase PCR components in separate tubes to allow for optimization
11636103001 PCR Master
P4600 ReadyMix Taq PCR Reaction Mix with MgCl2
D4309 REDTaq® DNA Polymerase Taq for routine PCR with inert dye, 10X buffer included
D8312 REDTaq® Genomic DNA Polymerase with MgCl2
D2812 REDTaq® Genomic DNA Polymerase without MgCl2
R2523 REDTaq® ReadyMix PCR Reaction Mix Complete PCR reagent with standard Taq DNA Polymerase and inert dye
D6063 REDTaq® SuperPak DNA Polymerase Taq for routine PCR with inert dye; with 10X buffer & dNTP mix
D1806 Taq DNA Polymerase from Thermus aquaticus with 10× PCR reaction buffer containing MgCl2
D4545 Taq DNA Polymerase from Thermus aquaticus with 10× PCR reaction buffer without MgCl2
TAQL-RO Taq DNA Polymerase, 1 U/μl
TAQ-RO Taq DNA Polymerase, 5 U/μl
TAQN-RO Taq DNA Polymerase, dNTPack 5 U/βl
TAQNL-RO Taq DNA Polymerase (1 U/μl), dNTPack
D5938 Taq SuperPak DNA Polymerase with MgCl2