Nucleic Acid Electrophoresis

Agarose & Acrylamide

Agarose

Sigma-Aldrich offers a range of molecular biology grade agarose products from routine use to wide range and low melt point agarose. Each lot of products is rigorously tested to ensure reproducibility from one lot to the next. Use the following table to select the best product for your application. For more information about agarose electrophoresis, see the Molecular Biology Handbook.

Product No. Product Name Recommended DNA size range Specific applications Gelling Temp
(°C)
Melting Temp
(°C)
EEO Moisture Gel Strength (g/cm2) Sulfate
A9539 Routine Use 0.5-25 kb analysis, purification 36 + 1.5   0.09-0.13 <10% >1200 1% gel <0.15%
A2929 Pulsed-field gel electrophoresis 10-2000 kb field inversion electrophoresis 42+ 1.5   0.04-0.07 <8% >2400 1.5% gel <0.10%
A3054 Pulsed-field sample prep N/A gel plugs, lysis and digestion of embedded DNA 26-30 <70 <0.10 <10% >400 1% gel <0.15%
A9414 Low melting 0.5-25 kb cloning and isolation from agarose 26-30 <65 <0.10 <10% >200 1% gel <0.10%
A7431 Wide range: standard (3:1) 50-1000 bp small NA fragments; strong flexible gels 32.5-38 <90 <0.13 <10% >1400 4% gel <0.15%


Pre-cast Agarose Gels

Pre-cast agarose gels are designed for high-resolution separation of nucleic acids, producing sharp DNA and RNA bands with low background fluorescence. Pre-cast gels are individually foil-sealed in plastic trays and can be electrophoresed right in the plastic tray. They provide the ultimate in convenience for nucleic acid agarose electrophoresis.

Product Name Product No.
1.0% Agarose in TBE buffer with 0.5 µg/ml Ethidium bromide 8-well (portrait orientation) P5472
1.0% Agarose in TBE buffer with 0.5 µg/ml Ethidium bromide 20-well (landscape orientation) P5722
1.0% Agarose in TBE buffer with 0.5 µg/ml Ethidium bromide 24-well (2x12-well portrait orientation) P5972
4.0% Agarose in TBE buffer with 0.5 µg/ml Ethidium bromide 24-well (2x12-well portrait orientation) P6097
1.25% Agarose in MOPS for RNA electrophoresis 8-well (portrait orientation) P6222


Acrylamide

Acrylamide is used for both protein and nucleic acid electrophoresis.

Product Name Product No.
Acrylamide, for molecular biology, minimum 99% A9099
Acrylamide, analytical standard  23701
Acrylamide, certified reference material, TraceCERT® 08267
Acrylamide, for Northern and Southern blotting, powder blend  01696
Acrylamide, purum, ≥98.0% (GC)  01700
Acrylamide, for electrophoresis, ≥99% (HPLC), powder  A3553