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Nucleic Acid Electrophoresis

Dyes & Stains

Dyes and Loading Buffers
 

Product Name Product #
Gel Loading Solution G2526
Gel Loading Solution Type I G7654
RNA Sample Loading Buffer Without Ethidium bromide R1386
RNA Sample Loading Buffer With Ethidium bromide R4268
Bromphenol Blue sodium salt B5525
Bromphenol Blue-Xylene Cyanole Dye solution B3269
Xylene Cyanole FF X4126

For more information on selecting appropriate dyes, please visit the Molecular Biology Handbook

 

Nucleic Acid Gel Stains

A wide range of nucleic acid stains for polyacrylamide and agarose are available including Nancy-520, Ethidium bromide, SYBR® Green I and II, methylene blue, DAPI, acridine orange and others. The table helps identify the stains, which can be used for various nucleic acid applications, and their detection limits by gel electrophoresis. Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. Nancy-520, along with SYBR® Green I and II have Inherently Safer Chemistry, compared to the standard use of ethidium bromide for staining. For more information see publication on Greener Alternatives.

 

Application Nancy-520 Ethidium bromide Methylene blue SYBR® Green I SYBR® Green II Pyronin Y DAPI Acridine Orange
DNA + + + +     + +
RNA   + +   + +   +
Fluorescent + +   + + + + +
Colorimetric     +     +    
Agarose + + + + +   + +
PAGE   + + + + +   +
Detection limits 0.5 ng DNA 1 ng DNA 5 ng DNA 60 pg DNA 0.5 ng RNA   0.5 µg DNA 50 ng DNA

 

Product Name Product No.
Nancy-520 01494
Ethidium bromide solution E1510
Methylene Blue hydrate M4159
SYBR® Green I nucleic acid gel stain S9430
SYBR® Green II RNA gel stain S9305
Pyronin Y P9172
DAPI D9542
Acridine Orange hemi(zinc chloride) salt A6014