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Nucleic Acid Electrophoresis
Agarose & Acrylamide
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Agarose and Acrylamide
Agarose
Sigma-Aldrich offers a range of molecular biology grade agarose products from routine use to wide range and low melt point agarose. Each lot of products is rigorously tested to ensure reproducibility from one lot to the next. Use the following table to select the best product for your application.
| Product # |
Product Name |
Recommended DNA size range |
Specific applications |
Gelling Temp
(°C) |
Melting Temp
(°C) |
EEO |
Moisture |
Gel Strength (g/cm2) |
Sulfate |
| A9539 |
Routine Use |
0.5-25 kb |
analysis, purification |
36 + 1.5 |
|
0.09-0.13 |
<10% |
>1200 1% gel |
<0.15% |
| A4718 |
High Resolution |
200-800 bp |
small NA fragments |
<35 |
<75 |
<0.05 |
<10% |
>300 3% gel |
|
| A2929 |
Pulsed-field gel electrophoresis |
10-2000 kb |
field inversion electrophoresis |
42+ 1.5 |
|
0.04-0.07 |
<8% |
>2400 1.5% gel |
<0.10% |
| A3054 |
Pulsed-field sample prep |
N/A |
gel plugs, lysis and digestion of embedded DNA |
26-30 |
<70 |
<0.10 |
<10% |
>400 1% gel |
<0.15% |
| A9414 |
Low melting |
0.5-25 kb |
cloning and isolation from agarose |
26-30 |
<65 |
<0.10 |
<10% |
>200 1% gel |
<0.10% |
| A2790 |
Wide range |
50-1000 bp |
small NA fragments |
<35 |
<65 |
<0.15 |
<10% |
>500 4% gel |
<0.15% |
| A7431 |
Wind range: standard (3:1) |
50-1000 bp |
small NA fragments; strong flexible gels |
32.5-38 |
<90 |
<0.13 |
<10% |
>1400 4% gel |
<0.15% |
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Pre-cast agarose gels
Pre-cast agarose gels are designed for high-resolution separation of nucleic acids, producing sharp DNA and RNA bands with low background fluorescence. Pre-cast gels are individually foil-sealed in plastic trays and can be electrophoresed right in the plastic tray. They provide the ultimate in convenience for nucleic acid agarose electrophoresis.
| Product Name |
Technical Bulletin |
Product # |
| 1.0% Agarose in TBE buffer with 0.5 µg/ml Ethidium bromide 8-well (portrait orientation) |
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P5472 |
| 1.0% Agarose in TBE buffer with 0.5 µg/ml Ethidium bromide 20-well (landscape orientation) |
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P5722 |
| 1.0% Agarose in TBE buffer with 0.5 µg/ml Ethidium bromide 24-well (2x12-well portrait orientation) |
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P5972 |
| 4.0% Agarose in TBE buffer with 0.5 µg/ml Ethidium bromide 8-well (portrait orientation) |
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P5597 |
| 4.0% Agarose in TBE buffer with 0.5 µg/ml Ethidium bromide 20-well (landscape orientation) |
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P5847 |
| 4.0% Agarose in TBE buffer with 0.5 µg/ml Ethidium bromide 24-well (2x12-well portrait orientation) |
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P6097 |
| 1.25% Agarose in MOPS for RNA electrophoresis 8-well (portrait orientation) |
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P6222 |
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Acrylamide
Acrylamide is used for both protein and nucleic acid electrophoresis. The following table can be used to determine the appropriate acrylamide:bis acrylamide concentration for your application.
| Product Name |
Technical Bulletin |
Product # |
| Acrylamide, for molecular biology, minimum 99% |
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A9099 |
| Acrylamide, for mutation detection |
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A5934 |
| Gel Concentration (% w/v) |
Effective range of separation (bp) |
Xylene Cyanol (bp) |
Bromophenol Blue (bp) |
| 3.5 |
1000-2000 |
460 |
100 |
| 5.0 |
50-500 |
260 |
65 |
| 8.0 |
60-400 |
160 |
45 |
| 12.0 |
40-200 |
70 |
20 |
| 15.0 |
25-150 |
60 |
15 |
| 20.0 |
6-100 |
45 |
12 |
Acrylamide:bis-acrylamide ratio 29.1, 1X TBE buffer
Size of the double stranded DNA, which comigrates with the dye.
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