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Better...Faster...Stronger!

Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for higher voltage runs, extremely rapid running times, and high-resolution band separation.

Resolve bands in minutes
Run gels 2-3x faster than in TBE/TAE
Sharper bands in less time
Compatible with pre-cast gels
Voltage less limited by heat generation

For best results, prepare and run your agarose gel with Bionic Buffer. Alternatively, Bionic Buffer is compatible with standard pre-cast gels or freshly made TBE and TAE gels.

Note A voltage of 150 V is recommended for small electrophoresis chambers while a mid to large apparatus may be run at 200-250 V. It is recommended that the user test their chamber to determine optimum voltage.:

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Order Information

Product Code	Product Name	Size
B6185	Bionic Buffer, 10x Buffer	100 mL 1 L 4 L 10 L

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Sample Data

High Resolution Band Separation in Less Time!

Figure 1. Lanes 1, 4 & 7: 50 - 3,000 bp marker; Lanes 2 & 5: 100 - 1,000 bp marker; Lanes 3 & 6: 50 - 2,000 bp marker. PCR markers were loaded on 0.8% agarose gels prepared with (1x) Bionic Buffer or (1x) TBE Buffer. Bionic Buffer ran at its optimal voltage of 250 volts; TBE was run at 100 volts. In only 10 minutes, Bionic Buffer allows for better and sharper band resolution than traditional TBE.

Works great with pre-cast gels!

Figure 2. This is a 1% TBE pre-cast gel (P5472) electrophoresed with 1x Bionic Buffer for 10 minutes at 250 volts. There is excellent band resolution and separation in a very short time with the pre-cast gel. This gel was loaded and then run with no equilibration time. Gel was loaded as follows: Lane 1: 100-1000 bp DNA ladder (D3687); Lane 2: 50-2000 bp PCR Marker (P9577); Lanes 3,4: 500-10,000 bp 1 KB ladder (D3937).

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