PCR/Amplification

Epoch Biosciences


PCR Home Page Routine Amplification | Human Genomic DNA | Long & Accurate PCR Reagents | Hot Start PCR | RT-PCR | Quantitative PCR | Specialty Enzymes | Ultrapure Nucleotides | PCR Reaction Components | PCR Lab Equipment | DNA/RNA Molecular Weight Markers | Whole Genome Amplification


Sigma-Aldrich has teamed with Epoch Biosciences to create an optimized PCR reagent for the MGB Eclipse™ Probe Systems for both Gene Expression Profiling and SNP Analysis. The PCR reagents are pre-formulated with necessary buffers and deoxynucleotides and when combined with JumpStart™ Taq DNA Polymerase creates a ready-to-use 2X PCR master mix.

 

  • The ideal reagent for high performance gene expression analysis and general sequence detection using MGB Eclipse Probe Systems.
  • Scalable for different reaction volumes and flexible for optional use of UNG.
  • Prevents non-specific product formation when combined with the hot start mechanism of the JumpStart Taq antibody and allows assembled PCR reactions to be placed at room temperature for up to 2 hours without compromising the performance.
  • Can save a significant amount of preparation time, reduce the risk of contamination from multiple pipetting steps, and provide consistent batch-to-batch and reaction-to-reaction performance when performing large numbers of PCR reactions.

 


Product Name Product # 
MGB Eclipse™ PCR Reagent Kit M4568
MGB Eclipse™ PCR Reagent Kit for SNPs M4693

MGB Eclipse™ PCR Reagent Kit, M4568

MGB Eclipse™ PCR Reagent Kit is optimized for quantitative PCR mediated Gene Expression Analysis in conjunction with MGB Eclipse™ Probe Systems. It is pre-formulated with necessary buffers and deoxynucleotides and when combined with JumpStart™ Taq DNA Polymerase creates a ready-to-use 2X PCR master mix. MGB Eclipse PCR Reagent Kit also contains dUTP for the optional addition of uracil-N-glycosylase (UNG) to prevent carry-over contamination from previous target amplification. MGB Eclipse PCR Reagent Kit can be used for all real-time PCR applications with the exception of MGB Eclipse Probe Systems designed for allelic discrimination or single nucleotide polymorphism analysis. For such applications, MGB Eclipse PCR Reagent Kit for SNPs Product Code M4693 is recommended. Once the 2X master mix is prepared, combine and run with probes, primers, and template according to the protocol provided with your MGB Eclipse Probe System from Epoch Biosciences.


MGB Eclipse™ PCR Reagent Kit for SNPs, M4693

MGB Eclipse PCR Reagent Kit for SNPs is optimized for allelic discrimination or single nucleotide polymorphism analysis with the MGB Eclipse™ Probe System. It is pre-formulated with necessary buffers and deoxynucleotides and when combined with JumpStart™ Taq DNA Polymerase creates a ready to use 2X PCR master mix. MGB Eclipse PCR Reagent Kit for SNPs also contains dUTP for the optional addition of uracil-N-glycosylase (UNG) to prevent carry-over contamination from previous target amplification. Once the 2X master mix is prepared, combine with probes, primers, and template and run according to the protocol provided with your MGB Eclipse Probe System from Epoch Biosciences.