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PCR/Amplification

PCR Calculator

Performing calculations for large scale PCR reactions can be cumbersome and tedious. Ensure your success of scaled up reactions by using the PCR MasterMix Calculator. This online tool will calculate the amounts of components needed to create your PCR MasterMix.



Quickly calculate the amount of components needed to create your PCR MasterMix.


SIGMA-ALDRICH
PCR MasterMix Calculator
 
Composition of PCR reaction PCR MasterMix Formulation
for PCR reactions
 
  Template DNA µl   Template DNA µl
PCR Buffer µl PCR Buffer µl
Forward Primer µl Forward Primer µl
Reverse Primer µl Reverse Primer µl
dNTP mix µl dNTP mix µl
DNA Polymerase µl DNA Polymerase µl
PCR grade Water µl PCR grade Water µl
Total Number of Reactions  
  Total PCR Reaction Volume µl
  TOTAL VOLUME µl