|
Many of today's PCR based applications require longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase. Cloning and expression experiments, cDNA analysis and array work can be improved by using long and accurate amplification over routine amplification.
Long and Accurate (LA) amplification is achieved by combining a thermostable polymerase with a second polymerase exhibiting a 3'—>5' exonuclease activity. The exonuclease, or proofreading, activity repairs terminal misincorporations. This repair allows for greater read lengths and increased fidelity.
Sigma Aldrich brings you a complete line of Long and Accurate products for DNA research. Our AccuTaq LA and KlenTaq LA enzymes and blends provide increased yield, higher processivity for greater lengths, and proofreading activity for increased fidelity over other long and accurate enzymes.
AccuTaq™ LA DNA Polymerase and REDAccuTaq™ LA DNA Polymerase KlenTaq® LA DNA Polymerase, KlenTaq DV ReadyMix™, and RED KlenTaq DV ReadyMix JumpStart™ AccuTaq LA DNA Polymerase and JumpStart REDAccuTaq LA DNA Polymerases JumpStart KlenTaq LA DNA Polymerase Restorase™ DNA Polymerase
AccuTaq LA DNA Polymerase (D8045) and REDAccuTaq LA DNA Polymerase (D4812)
A blend of Sigma's quality Taq DNA polymerase with a 3'—>5' proofreading exonuclease. This mix provides increased yield and 6.5X greater fidelity over standard Taq DNA polymerase.
- Generate amplicons up to 20 kb on complex, genomic templates or 40 kb on less complex DNA
- Increased yield over competitors' long and accurate enzymes
- REDAccuTaq allows for quick recognition of addition and mixing as well as direct loading to an agarose gel
 |
Amplification of 25-40 kb lambda DNA template using AccuTaq LA DNA Polymerase Lane 1: 25 kb Lane 2: 31 kb Lane 3: 36 kb Lane 4: 40 kb Lane M1: Lambda Hind III marker Lane M2: PCR 100 bp ladder
|
|