Long & Accurate PCR

Many of today's PCR based applications require longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase. Cloning and expression experiments, cDNA analysis and array work can be improved by using long and accurate amplification over routine amplification.

Long and Accurate (LA) amplification is achieved by combining a thermostable polymerase with a second polymerase exhibiting a 3'—>5' exonuclease activity. The exonuclease, or proofreading, activity repairs terminal misincorporations. This repair allows for greater read lengths and increased fidelity.

Sigma Aldrich brings you a complete line of Long and Accurate products for DNA research. Our AccuTaq LA and KlenTaq LA enzymes and blends provide increased yield, higher processivity for greater lengths, and proofreading activity for increased fidelity over other long and accurate enzymes.

AccuTaq™ LA DNA Polymerase and REDAccuTaq™ LA DNA Polymerase
KlenTaq^® LA DNA Polymerase, KlenTaq DV ReadyMix™, and RED KlenTaq DV ReadyMix
JumpStart™ AccuTaq LA DNA Polymerase and JumpStart REDAccuTaq LA DNA Polymerases
JumpStart KlenTaq LA DNA Polymerase
Restorase™ DNA Polymerase

AccuTaq LA DNA Polymerase (D8045) and
REDAccuTaq LA DNA Polymerase (D4812)

A blend of Sigma's quality Taq DNA polymerase with a 3'—>5' proofreading exonuclease. This mix provides increased yield and 6.5X greater fidelity over standard Taq DNA polymerase.

• Generate amplicons up to 20 kb on complex, genomic templates or 40 kb on less complex DNA
• Increased yield over competitors' long and accurate enzymes
• REDAccuTaq allows for quick recognition of addition and mixing as well as direct loading to an agarose gel

Amplification of 25-40 kb lambda DNA template using AccuTaq LA DNA Polymerase Lane 1: 25 kb Lane 2: 31 kb Lane 3: 36 kb Lane 4: 40 kb Lane M1: Lambda Hind III marker Lane M2: PCR 100 bp ladder

Click here for more information on JumpStart AccuTaq LA and JumpStart REDAccuTaq LA DNA polymerase

JumpStart KlenTaq LA DNA Polymerase (D0816)

An antibody inactivated formulation of KlenTaq LA delivers the high yield and improved processivity of KlenTaq with the specificity of a hot start enzyme.

• Greater specificity with antibody inactivation over standard Taq or chemically inactivated enzymes
• Increased yields compared to other hot start polymerases, especially when amplifying templates with high GC content
  

JumpStart KlenTaq LA and competitors' high performance enzymes were used to amplify an 890 bp amplicon containing 80% overall G-C content from total human genomic DNA. Reactions were run according to supplier's recommendations both without (Lanes 1-5) and with (Lanes 6-10) suggested PCR enhancers.
	Run using supplier's recommended conditions Lane M: PCR Marker Lane 1: Sigma Taq Lane 2: Supplier S, enzyme H Lane 3: Supplier Q Lane 4: Supplier S, enzyme Y Lane 5: JumpStart KlenTaq LA
	Run using supplier's recommended conditions and enhancers Lane M: PCR Marker Lane 6: Taq DNA polymerase witn 1M betaine Lane 7: Supplier S, enzyme H with 4% DMSO Lane 8: Supplier Q with 1X Q solution Lane 9: Supplier S, enzyme Y with 4% DMSO Lane 10: JumpStart KlenTaq LA with 1 M betaine

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