Real-time or quantitative PCR (qPCR) allows quantification of starting amounts of DNA, cDNA, or RNA templates. qPCR is based on the detection of a fluorescent reporter molecule that increases as PCR product accumulates with each cycle of amplification. Fluorescent reporter molecules include dyes that bind double-stranded DNA (i.e. SYBR Green I) or sequence-specific probes (i.e. Molecular Beacons or TaqMan® Probes).
qPCR exceeds the limitations of traditional end-point PCR methods by allowing either absolute or relative quantification of PCR product at the end of each cycle. The ability has greatly enhanced several areas of research including gene expression analysis and genotyping assays.
Improve your qPCR results today. From probe based to SYBR® based qPCR, our products are compatible with a variety of instruments and optimized to help you achieve superior results!
