Probe-based qPCR

Probe based qPCR relies on the sequence–specific detection of a desired PCR product. Unlike SYBR^® based qPCR methods that detect all double-stranded DNA, probe based qPCR utilizes a fluorescent-labeled target-specific probe resulting in increased specificity and sensitivity. Additionally, a variety of fluorescent dyes are available so that multiple primers can be used to simultaneously amplify many sequences.

• Ideal for high throughput. ReadyMixes contain all necessary components for qPCR you simply add the fluorescent detection chemistry, primers, and template.
• Specific – JumpStart™ Taq antibody prevents non–specific product formation through it hot-start mechanism.
• Maximum flexibility in detection method, since no detection method has been incorporated into formulation.
• Optimized formulations with the addition of dUTP to facilitate carry-over prevention from previous PCR.

Product Name	Technical Bulletin	Product No.
JumpStart Taq ReadyMix™ for High Throughput qPCR		D6442
JumpStart Taq ReadyMix for qPCR		D7440
JumpStart Taq ReadyMix with dUTP		D9191

Mechanism of probe based protocol

Figures 1 & 2. Superior Amplification Efficiency with Lower C_T.

Sigma-Aldrich Teams with Epoch Biosciences
Sigma-Aldrich has teamed with Epoch Biosciences to create an optimized PCR reagent for the MGB Eclipse™ Probe Systems for both Gene Expression Profiling and SNP Analysis. The PCR reagents are pre-formulated with necessary buffers and deoxynucleotides and when combined with JumpStart™ Taq DNA Polymerase creates a ready-to-use 2X PCR master mix.

Back to Quantitative PCR home page.

Routine Amplification | Human Genomic DNA | Long & Accurate PCR Reagents | Hot Start PCR | RT-PCR | Quantitative PCR | Specialty Enzymes | Ultrapure Nucleotides | PCR Reaction Components | PCR Lab Equipment | DNA/RNA Molecular Weight Markers | Whole Genome Amplification