Probe based qPCR relies on the sequence–specific detection of a desired PCR product. Unlike SYBR® based qPCR methods that detect all double-stranded DNA, probe based qPCR utilizes a fluorescent-labeled target-specific probe resulting in increased specificity and sensitivity. Additionally, a variety of fluorescent dyes are available so that multiple primers can be used to simultaneously amplify many sequences.
|Product Name||Technical Bulletin||Product No.|
|JumpStart Taq ReadyMix™ for High Throughput qPCR||D6442|
|JumpStart Taq ReadyMix for qPCR||D7440|
|JumpStart Taq ReadyMix with dUTP||D9191|
Figures 1 & 2. Superior Amplification Efficiency with Lower CT.
Sigma-Aldrich Teams with Epoch Biosciences
Sigma-Aldrich has teamed with Epoch Biosciences to create an optimized PCR reagent for the MGB Eclipse™ Probe Systems for both Gene Expression Profiling and SNP Analysis. The PCR reagents are pre-formulated with necessary buffers and deoxynucleotides and when combined with JumpStart™ Taq DNA Polymerase creates a ready-to-use 2X PCR master mix.
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