qPCR Technical Guide

Sigma recognizes the constant evolution of qPCR applications and kits. To support our commitment of increasing your qPCR success, we have provided the qPCR Technical Guide to assist you with understanding the field. Within the qPCR Technical Guide you will find the necessary information to make the appropriate choices and decisions before beginning your experiments.

(3.28 Mb PDF)

Table of Contents Quantitative PCR: How does it work? qPCR Detection Methods Primer and Probe Design Instrumentation qPCR Applications Guide Optimizing qPCR qPCR Reagent Selection Table Troubleshooting Appendix 1: Traits of Commonly Used Fluorophores Appendix 2: How to Optimize Your Quantitative Real-Time RT-PCR References

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