Quantitative RT-PCR

Quantitative Reverse Transcriptase PCR (QRT–PCR) provides a highly sensitive method for the quantitative analysis of gene expression. Sigma's QRT–PCR ReadyMixes combine the advantages of Enhanced Avian Reverse Transcriptase (eAMVTM RT) and JumpStart Taq in a convenient ReadyMix designed for either probe based or SYBR based QRT–PCR. Regardless of difficult secondary structures or detection chemistry, Sigma's QRT–PCR ReadyMixes are specially formulated to help you achieve superior results.

• Increased Thermostability and Processivity
  eAMV reverse transcriptase has the ability to transcribe through difficult secondary structures at elevated temperature (up to 65 °C).
• Greater Specificity & Sensitivity
  Inactivated by a taq–directed antibody, Sigma's JumpStart Taq enzyme decreases non–specific amplification while enhancing target yield.
• Maximum Versatility
  Probe based or SYBR based detection, our RT kits are optimized to help you achieve superior results and compatible with both tube/plate based instruments and capillary based instruments.

Product Name	Technical Bulletin	Product #
SYBR Green Quantitative RT–PCR		QR0100
Quantitative RT–PCR ReadyMix for probe based applications		QR0200

Figure 1. Sensitive QRT–PCR using Sigma's SYBR Green QRT–PCR Kit.

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