SYBR Green I, a commonly used fluorescent DNA binding dye, binds all double–stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. Specificity of Sigma's SYBR based QPCR detection is greatly enhanced by the incorporation of a hot–start mediated taq polymerase, JumpStart Taq.
- Convenient – Delivers the benefits of antibody–inactivated hot–start PCR with SYBR Green detection in a ReadyMix ideal for high throughput applications; only primers and template are required.
- Specific – JumpStart Taq antibody prevents non–specific product formation through its hot–start mechanism.
- Flexible – SYBR Green JumpStart Taq ReadyMixes for SYBR based QPCR are formulated with MgCl2 or packaged with a separate vial for ease of optimization. Additionally, our ReadyMixes are compatible with tube– and plate–based instruments.
| Product Name |
Technical Bulletin |
Product # |
| SYBR Green JumpStart Taq ReadyMix for High Throughput QPCR |
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S9194 |
| SYBR Green JumpStart Taq ReadyMix for QPCR |
|
S4438 |
| SYBR Green JumpStart Taq ReadyMix without MgCl2 |
|
S5193 |
Mechanism of SYBR Green–based detection
 |
Figure 1. Linearity of pBAC–2cp 10–fold dilutions with SYBR Green JumpStart Taq ReadyMix. |
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Figure 2. CT Values for Lambda Amplicon using SYBR Green I JumpStart Taq ReadyMix.
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