SYBR Green based qPCR

SYBR Green I, a commonly used fluorescent DNA binding dye, binds all double–stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. Specificity of Sigma's SYBR based QPCR detection is greatly enhanced by the incorporation of a hot–start mediated taq polymerase, JumpStart Taq.

• Convenient – Delivers the benefits of antibody–inactivated hot–start PCR with SYBR Green detection in a ReadyMix ideal for high throughput applications; only primers and template are required.
• Specific – JumpStart Taq antibody prevents non–specific product formation through its hot–start mechanism.
• Flexible – SYBR Green JumpStart Taq ReadyMixes for SYBR based QPCR are formulated with MgCl₂ or packaged with a separate vial for ease of optimization. Additionally, our ReadyMixes are compatible with tube– and plate–based instruments.

Product Name	Technical Bulletin	Product #
SYBR Green JumpStart Taq ReadyMix for High Throughput QPCR		S9194
SYBR Green JumpStart Taq ReadyMix for QPCR		S4438
SYBR Green JumpStart Taq ReadyMix without MgCl2		S5193

Mechanism of SYBR Green–based detection

	Figure 1. Linearity of pBAC–2cp 10–fold dilutions with SYBR Green JumpStart Taq ReadyMix.
	Figure 2. CT Values for Lambda Amplicon using SYBR Green I JumpStart Taq ReadyMix.

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