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Our RED enzymes offer speed and simplicity
for every PCR application!
The direct load capability of Sigma's patented line of RED enzymes provides a time-saving reduction in steps between PCR and agarose gel analysis. RED enzymes are a unique blend of our quality DNA polymerases combined with an inert red dye. In addition to reducing transfer steps, this dye provides visual confirmation of proper mixing of the PCR reaction components and ensures uniformity between samples. This Red formulation has been incorporated into all of our PCR product areas — Routine PCR Amplification, Hot Start PCR, Long and Accurate, and Genomic DNA Amplification.
For even greater simplicity and time savings, use our RED enzyme ReadyMixes. These mixes are provided as a 2x optimized blend of RED enzyme, 99% pure dNTPs, MgCl2, and PCR buffer. Simply add primers and template to the final reaction volume and begin cycling.
RED enzymes provide:
- A reduction in steps for high throughput applications
- Visual confirmation that enzyme has been added and proper mixing has occurred
- A variety of options to meet all areas of PCR research
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No loading buffers or tracking dyes required
Samples may be added directly to an agarose gel after PCR amplification without the addition of a loading buffer or tracking dye. The dye in REDTaq acts as a tracking dye migrating at approximately the same rate as a 125 bp fragment.
"Sigma's JumpStart™ REDTaq ReadyMix™ PCR Reaction Mix has helped me generate great data and quickly! It gives excellent specificity and yield when applied to high throughput genetic screening of small amounts of mouse-tail tissue. The fewer number of steps in the PCR procedure result from having the red tracking dye, buffer and dNTPs already in the mix, cutting the process time in half."
Andrew J. Paterson,
University of Alabama at Birmingham
Medicine/Endocrinology, Diabetes and Metabolism
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Product Area
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Product Code |
Product Name
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Package Size
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| Routine Amplification |
D4309 |
REDTaq DNA Polymerase with 10X Reaction Buffer containing MgCl2 |
50 units
250 units
1000 units
2500 units |
| R2523 |
REDTaq ReadyMix PCR Reaction Mix |
20 reactions
100 reactions |
| D6063 |
REDTaq Superpak DNA Polymerase |
50 units
250 units |
| Hot Start |
D8187 |
JumpStart REDTaq DNA Polymerase |
50 units
250 units
2500 units |
| P0982 |
JumpStart REDTaq ReadyMix PCR Reaction Mix |
20 reactions
100 reactions |
| P1107 |
JumpStart REDTaq ReadyMix for High Throughput PCR |
100 reactions
400 reactions |
| D1313 |
JumpStart REDAccuTaq LA DNA Polymerase |
50 units
250 units |
| Long and Accurate |
D4812 |
REDAccuTaq LA DNA Polymerase |
50 units
250 units
2500 units |
| D1816 |
RED Klentaq DV ReadyMix |
20 reactions
100 reactions |
| Genomic DNA |
D8312 |
REDTaq Genomic DNA Polymerase |
50 units
250 units
1000 units
2500 units |
| D2812 |
REDTaq Genomic DNA Polymerase without MgCl2 |
50 units
250 units
1000 units
2500 units |
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