REDTaq

Our RED enzymes offer speed and simplicity
for every PCR application!

The direct load capability of Sigma's patented line of RED enzymes provides a time-saving reduction in steps between PCR and agarose gel analysis. RED enzymes are a unique blend of our quality DNA polymerases combined with an inert red dye. In addition to reducing transfer steps, this dye provides visual confirmation of proper mixing of the PCR reaction components and ensures uniformity between samples. This Red formulation has been incorporated into all of our PCR product areas — Routine PCR Amplification, Hot Start PCR, Long and Accurate, and Genomic DNA Amplification.

For even greater simplicity and time savings, use our RED enzyme ReadyMixes. These mixes are provided as a 2x optimized blend of RED enzyme, 99% pure dNTPs, MgCl2, and PCR buffer. Simply add primers and template to the final reaction volume and begin cycling.

RED enzymes provide:

A reduction in steps for high throughput applications
Visual confirmation that enzyme has been added and proper mixing has occurred
A variety of options to meet all areas of PCR research

No loading buffers or tracking dyes required

Samples may be added directly to an agarose gel after PCR amplification without the addition of a loading buffer or tracking dye. The dye in REDTaq acts as a tracking dye migrating at approximately the same rate as a 125 bp fragment.

"Sigma's JumpStart™ REDTaq ReadyMix™ PCR Reaction Mix has helped me generate great data and quickly! It gives excellent specificity and yield when applied to high throughput genetic screening of small amounts of mouse-tail tissue. The fewer number of steps in the PCR procedure result from having the red tracking dye, buffer and dNTPs already in the mix, cutting the process time in half."

Andrew J. Paterson,
University of Alabama at Birmingham
Medicine/Endocrinology, Diabetes and Metabolism

Product Area	Product Code	Product Name	Package Size
Routine Amplification	D4309	REDTaq DNA Polymerase with 10X Reaction Buffer containing MgCl2	50 units 250 units 1000 units 2500 units
	R2523	REDTaq ReadyMix PCR Reaction Mix	20 reactions 100 reactions
	D6063	REDTaq Superpak DNA Polymerase	50 units 250 units
Hot Start	D8187	JumpStart REDTaq DNA Polymerase	50 units 250 units 2500 units
	P0982	JumpStart REDTaq ReadyMix PCR Reaction Mix	20 reactions 100 reactions
	P1107	JumpStart REDTaq ReadyMix for High Throughput PCR	100 reactions 400 reactions
	D1313	JumpStart REDAccuTaq LA DNA Polymerase	50 units 250 units
Long and Accurate	D4812	REDAccuTaq LA DNA Polymerase	50 units 250 units 2500 units
Long and Accurate	D1816	RED Klentaq DV ReadyMix	20 reactions 100 reactions
Genomic DNA	D8312	REDTaq Genomic DNA Polymerase	50 units 250 units 1000 units 2500 units
	D2812	REDTaq Genomic DNA Polymerase without MgCl₂	50 units 250 units 1000 units 2500 units

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