The study of gene expression, at both the RNA and protein levels, often begins with the assay of low abundance transcripts and/or transcripts containing difficult secondary structure. These messages exceed the capabilities of most reverse transcriptases. Sigma's RT-PCR kits, based on the Enhanced Avian Reverse Transcriptase are the most effective tool for reproducible reverse transcription of low abundance messages and messages with difficult secondary structure, and for subsequent high fidelity amplification of the cDNA product.

Different poly (A)+ RNA were used as a template in a two-step RT-PCR.
Lane 1 Lane 2,3 Lane 4,5 Lane 6,7 Lane 8,9 Lane 10,11	1 kb Ladder 2 kb Pol 3.5 kb Pol 5.3 kb TSC-2 6.8 kb Pol 8.9 kb APC

RT-PCR was performed on human phospholipase A2 (low abundance RNA,
lane 2-7) and human HPRT RNA (a medium abundance RNA, lane 8-13) using Sigma's Enhanced Avian RT, RNase
H-reduced AMV RT and RNase
H-minus M-MLV RT.

Enhanced Avian HS RT-PCR kits

Greater transcription lengths - up to 14 kb
Detects low abundance mRNA
Improved specificity and yield with JumpStart™ AccuTaq™ LA DNA polymerase
Flexibility with One-Step & Two-Step Protocols
HSRT20
HSRT100

Enhanced Avian First Strand Synthesis Kit

Detects low abundance mRNA
Optimum for transcribing through difficult secondary structure
The resulting cDNA is ready for PCR amplification

Enhanced Avian Reverse Transcriptase

Detects low abundance mRNA
Best enzyme on the market for transcribing through difficult secondary structure
More robust at elevated temperatures, up to 65°C

AMV Reverse Transcriptase

M-MLV Reverse Transcriptase

JumpStart™ RED HT RT-PCR Kit

RT PCR