Description:
The Chloroplast Isolation Kit provides a quick and efficient procedure to isolate intact chloroplasts from plant leaves. Intact chloroplasts are the best starting material for studies of chloroplastic processes such as carbon assimilation, electron flow and phosphorylation, metabolic transport, or protein targeting. The chloroplast fraction can be further extracted to obtain membrane, stroma, or thylakoid proteins as well as chloroplastic DNA and RNA.
The chloroplast isolation method includes mechanical cell wall and membrane breakage, removal of cell debris and unbroken leaf tissue by filtration, collection of total cell chloroplasts by centrifugation, and separation of intact from broken chloroplasts using a Percoll® layer or gradient. The Chloroplast Isolation Kit has been tested for use with spinach, pea, lettuce, cabbage, mangold, and tobacco.
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