Protoplasts are cells of plants, fungi, or bacteria in which the cell wall has been removed, but the plasma membrane is intact. Among the many uses for these “naked” cells is transient or permanent genetic transformation by introduction of trans-gene DNA, somatic hybridization by protoplast fusion of species or subspecies resistant to traditional cross-breeding, and isolation of sub-cellular organelles. We offer a number of enzyme products to break down the cell wall. Individual hydrolytic enzymes include cellulases, hemicellulase, pectinase, and pectolase. Some, such as pectinase from Rhyzopus sp. or DriselaseTM are crude extracts with several specificities, while two of our thermostable cellulases are nearly pure recombinant preparations. Listed also are several reagents necessary for handling protoplasts, including the vitamin mixture for Kao and Michayluk culture medium, fluorescein diacetate for determinate of protoplast viability, and a specially formulated plant protoplast digest/wash solution.