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Life Science > Molecular Biology > Plant Biotechnology > Tissue Culture Protocols > Classic Plant Media
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Plant Tissue Culture Protocols
Classic Plant Media | ||
| Product No. | Description |
| C1416 | Chu (N6) basal salt mixture |
| D6162 | DKW/Juglans basal salt mixture |
| G5768 | Gamborg's B-5 basal salt mixture |
| G5893 | Gamborg's B-5 basal salt mixture with minimal organics |
| H2395 | Hoagland's No. 2 basal salt mixture |
| M6774 | McCown's woody plant basal salt mixture |
| M5524 | Murashige and Skoog basal salt mixture (MS) |
| Q3376 | Quoirin and Lepoivre basal salt mixture |
| S6765 | Schenk and Hildebrandt basal salt mixture |
| W0876 | White's basal salt mixture |
| Component (mg/L) | C1416 | D6162 | G5768 | G5893 | H2395 | M6774 | M5524 | Q3376 | S6765 | W0876 |
| Aluminum chloride • 6H2O | ||||||||||
| Ammonium nitrate | 1416.0 | 400.0 | 1650.0 | 400.0 | ||||||
| Ammonium phosphate monobasic | 115.03 | 300.0 | ||||||||
| Ammonium sulfate | 463.0 | 134.0 | 134.0 | |||||||
| Boric acid | 1.6 | 4.8 | 3.0 | 3.0 | 2.86 | 6.2 | 6.2 | 6.2 | 5.0 | 1.5 |
| Calcium chloride anhydrous | 125.33 | 112.5 | 113.24 | 113.24 | 72.5 | 332.2 | 151.0 | |||
| Calcium nitrate | 1367.0 | 656.4 | 386.0 | 833.77 | 200.0 | |||||
| Calcium phosphate tribasic | ||||||||||
| Cobalt chloride • 6H2O | 0.025 | 0.025 | 0.025 | 0.025 | 0.1 | |||||
| Cupric sulfate • 5H2O | 0.25 | 0.025 | 0.025 | 0.08 | 0.25 | 0.025 | 0.025 | 0.2 | ||
| Na2-EDTA | 37.25 | 45.4 | 37.3 | 37.25 | 37.3 | 37.26 | 37.3 | 20.0 | ||
| ZnNa2-EDTA | ||||||||||
| Ferric chloride • 6H2O | ||||||||||
| Ferric citrate | ||||||||||
| Ferric sulfate | 2.5 | |||||||||
| Ferric tartrate • 2H2O | 5.32 | |||||||||
| Ferrous sulfate • 7H2O | 27.85 | 33.8 | 27.8 | 27.85 | 27.8 | 27.8 | 27.8 | 15.0 | ||
| Magnesium sulfate | 90.37 | 361.49 | 122.09 | 122.09 | 240.76 | 180.7 | 180.7 | 175.79 | 195.4 | 360.0 |
| Manganese chloride • 4H2O | 1.81 | |||||||||
| Manganese sulfate • H2O | 3.33 | 33.5 | 10.0 | 10.0 | 22.3 | 16.9 | 0.76 | 10.0 | 5.04 | |
| Molybdenum trioxide | 0.016 | |||||||||
| Molybdic acid (sodium salt) • 2H2O | 0.39 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.1 | |||
| Nickel chloride • 6H2O | ||||||||||
| Nickel sulfate • 6H2O | 0.005 | |||||||||
| Potassium chloride | 65.0 | |||||||||
| Potassium iodide | 0.8 | 0.75 | 0.75 | 0.83 | 0.08 | 1.0 | 0.75 | |||
| Potassium nitrate | 2830.0 | 2500.0 | 2500.0 | 606.6 | 1900.0 | 1800.0 | 2500.0 | 80.0 | ||
| Potassium phosphate monobasic | 400.0 | 265.0 | 170.0 | 170.0 | 270.0 | |||||
| Potassium sulfate | 1559.0 | 990.0 | ||||||||
| Sodium nitrate | ||||||||||
| Sodium phosphate monobasic | 130.5 | 130.5 | 16.5 | |||||||
| Sodium sulfate | 200.0 | |||||||||
| Zinc nitrate • 6H2O | 17.0 | |||||||||
| Zinc sulfate • 7H2O | 1.5 | 2.0 | 2.0 | 0.22 | 8.6 | 8.6 | 8.6 | 1.0 | 2.67 | |
| Organics (mg/L) | C1416 | D6162 | G5768 | G5893 | H2395 | M6774 | M5524 | Q3376 | S6765 | W0876 |
| Activated charcoal | ||||||||||
| Adenine hemisulfate | ||||||||||
| Agar | ||||||||||
| 6-Benzylaminopurine (BA) | ||||||||||
| D-Biotin | ||||||||||
| 6gamma-gamma- Dimethylallylaminopurine |
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| Glycine (free base) | ||||||||||
| Indole-3-acetic acid | ||||||||||
| Indole-3-butyric acid | ||||||||||
| Kinetin | ||||||||||
| MES (free acid) | ||||||||||
| myo-Inositol | 100.0 | |||||||||
| alpha-Naphthaleneacetic acid | ||||||||||
| Nictotinic acid (free acid) | 1.0 | |||||||||
| Peptone | ||||||||||
| Pyridoxine • HCl | 1.0 | |||||||||
| Sucrose | ||||||||||
| Thiamine • HCl | 10.0 | |||||||||
| C1416 | D6162 | G5768 | G5893 | H2395 | M6774 | M5524 | Q3376 | S6765 | W0876 | |
| Grams of powder to prepare 1 L | 4.0 | 5.2 | 3.1 | 3.2 | 1.6 | 2.3 | 4.3 | 3.6 | 3.2 | 0.934 |
| pH 1 0.5 at room temperature | 4.1 | 4.1 | 4.0 | 4.0 | 4.7 | 3.9 | 3.9 | 3.9 | 4.2 | 4.7 |