Plant Tissue Culture Protocols

Murashige Skoog

Component (mg/L) M5524 M0654 M0529 M2909
Aluminum chloride • 6H2O        
Ammonium nitrate 1650.0 1650.0    
Ammonium phosphate monobasic        
Ammonium sulfate        
Boric acid 6.2   6.2 6.2
Calcium chloride anhydrous 332.2 332.2   332.2
Calcium nitrate        
Calcium phosphate tribasic        
Cobalt chloride • 6H2O 0.025   0.025 0.025
Cupric sulfate • 5H2O 0.025   0.025 0.025
Na2-EDTA 37.26   37.3 37.26
ZnNa2-EDTA        
Ferric chloride • 6H2O        
Ferric citrate        
Ferric sulfate        
Ferric tartrate • 2H2O        
Ferrous sulfate • 7H2O 27.8   27.8  
Magnesium sulfate 180.7 180.7   180.7
Manganese chloride • 4H2O        
Manganese sulfate • H2O 16.9   16.9 16.9
Molybdenum trioxide        
Molybdic acid (sodium salt) • 2H2O 0.25   0.25 0.25
Nickel chloride • 6H2O        
Nickel sulfate • 6H2O        
Potassium chloride        
Potassium iodide 0.83   0.83 0.83
Potassium nitrate 1900.0 1900.0   1900.0
Potassium phosphate monobasic 170.0 170.0   170.0
Potassium sulfate        
Sodium nitrate        
Sodium phosphate monobasic        
Sodium sulfate        
Zinc nitrate • 6H2O        
Zinc sulfate • 7H2O 8.6   8.6 8.6
Organics (mg/L) M5524 M0654 M0529 M2909
Activated charcoal        
Adenine hemisulfate        
Agar        
6-Benzylaminopurine (BA)        
D-Biotin        
6gamma-gamma-
Dimethylallylaminopurine
       
Glycine (free base)        
Indole-3-acetic acid        
Indole-3-butyric acid        
Kinetin        
MES (free acid)        
myo-Inositol        
alpha-Naphthaleneacetic acid        
Nictotinic acid (free acid)        
Peptone        
Pyridoxine • HCl        
Sucrose        
Thiamine • HCl        
  M5524 M0654 M0529 M2909
Grams of powder to prepare 1 L 4.3 n/a n/a 2.7
pH 1 0.5 at room temperature 3.9 4.3 3.2 3.9

 

Component (mg/L) M6899 M5519 M0404 M9274 M7024
Aluminum chloride • 6H2O          
Ammonium nitrate 1650.0 1650.0 1650.0 1650.0 1650.0
Ammonium phosphate monobasic          
Ammonium sulfate          
Boric acid 6.2 6.2 6.2 6.2 6.2
Calcium chloride anhydrous 332.2 332.2 332.2 332.2 332.2
Calcium nitrate          
Calcium phosphate tribasic          
Cobalt chloride • 6H2O 0.025 0.025 0.025 0.025 0.025
Cupric sulfate • 5H2O 0.025 0.025 0.025 0.025 0.025
Na2-EDTA 37.26 37.26 37.26 37.26 37.26
ZnNa2-EDTA          
Ferric chloride • 6H2O          
Ferric citrate          
Ferric sulfate          
Ferric tartrate • 2H2O          
Ferrous sulfate • 7H2O 27.8 27.8 27.8 27.8 27.8
Magnesium sulfate 180.7 180.7 180.7 180.7 180.7
Manganese chloride • 4H2O          
Manganese sulfate • H2O 16.9 16.9 16.9 16.9 16.9
Molybdenum trioxide          
Molybdic acid (sodium salt) • 2H2O 0.25 0.25 0.25 0.25 0.25
Nickel chloride • 6H2O          
Nickel sulfate • 6H2O          
Potassium chloride          
Potassium iodide 0.83 0.83 0.83 0.83 0.83
Potassium nitrate 1900.0 1900.0 1900.0 1900.0 1900.0
Potassium phosphate monobasic 170.0 170.0 170.0 170.0 170.0
Potassium sulfate          
Sodium nitrate          
Sodium phosphate monobasic         148.0
Sodium sulfate          
Zinc nitrate • 6H2O          
Zinc sulfate • 7H2O 8.6 8.6 8.6 8.6 8.6
Organics (mg/L) M6899 M5519 M0404 M9274 M7024
Activated charcoal          
Adenine hemisulfate         80.0
Agar       8000.0  
6-Benzylaminopurine (BA)          
D-Biotin          
6gamma-gamma-
Dimethylallylaminopurine
        30.0
Glycine (free base)   2.0   2.0  
Indole-3-acetic acid         0.3
Indole-3-butyric acid          
Kinetin          
MES (free acid)          
myo-Inositol 100.0 100.0 100.0 100.0 100.0
alpha-Naphthaleneacetic acid          
Nictotinic acid (free acid)   0.5 1.0 0.5  
Peptone          
Pyridoxine • HCl   0.5 1.0 0.5  
Sucrose       30,000 30,000
Thiamine • HCl 0.4 0.1 10.0 0.1 0.4
  M6899 M5519 M0404 M9274 M7024
Grams of powder to prepare 1 L 4.4 4.4 4.4 42.4 34.7
pH 1 0.5 at room temperature 3.9 3.9 3.8 5.0 3.9