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Plant Tissue Culture Protocols

Murashige Skoog
 
Basal Salts Mixtures
Media including Organics

 

 

Basal Salts Mixtures

   
M 5524
M 0654
M 0529
M 0153
M 2909
M 8900
M 8280 		 
Murashige and Skoog basal salt mixture (MS)
Murashige and Skoog basal salt macronutrient solution
Murashige and Skoog basal salt micronutrient solution
Murashige and Skoog modified basal salt mixture
Murashige and Skoog modified basal salt mixture
Murashige and Skoog modified basal salt mixture
Murashige and Skoog modified basal salt mixture
 
Component (mg/L) M 5524 M 0654 M 0529 M 0153 M 2909 M 8900 M 8280
Aluminum chloride • 6H2O              
Ammonium nitrate 1650.0 1650.0   825.0   825.0  
Ammonium phosphate monobasic              
Ammonium sulfate              
Boric acid 6.2   6.2 6.2 6.2 6.2 6.2
Calcium chloride anhydrous 332.2 332.2   166.1 332.2 332.2 332.2
Calcium nitrate              
Calcium phosphate tribasic              
Cobalt chloride • 6H2O 0.025   0.025 0.025 0.025 0.025 0.025
Cupric sulfate • 5H2O 0.025   0.025 0.025 0.025 0.025 0.025
Na2-EDTA 37.26   37.3 18.63 37.26 37.26 37.26
ZnNa2-EDTA              
Ferric chloride • 6H2O              
Ferric citrate              
Ferric sulfate              
Ferric tartrate • 2H2O              
Ferrous sulfate • 7H2O 27.8   27.8 13.9   27.8 27.8
Magnesium sulfate 180.7 180.7   90.35 180.7 180.7 180.7
Manganese chloride • 4H2O              
Manganese sulfate • H2O 16.9   16.9 16.9 16.9 16.9 16.9
Molybdenum trioxide              
Molybdic acid (sodium salt) • 2H2O 0.25   0.25 0.25 0.25 0.25 0.25
Nickel chloride • 6H2O              
Nickel sulfate • 6H2O              
Potassium chloride              
Potassium iodide 0.83   0.83 0.83 0.83 0.83 0.83
Potassium nitrate 1900.0 1900.0   950.0 1900.0 950.0 1900.0
Potassium phosphate monobasic 170.0 170.0   85.0 170.0 170.0 170.0
Potassium sulfate              
Sodium nitrate             1751.0
Sodium phosphate monobasic              
Sodium sulfate              
Zinc nitrate • 6H2O              
Zinc sulfate • 7H2O 8.6   8.6 8.6 8.6 8.6 8.6
Organics (mg/L) M 5524 M 0654 M 0529 M 0153 M 2909 M 8900 M 8280
Activated charcoal              
Adenine hemisulfate              
Agar              
6-Benzylaminopurine (BA)              
D-Biotin              
6gamma-gamma-
Dimethylallylaminopurine		              
Glycine (free base)              
Indole-3-acetic acid              
Indole-3-butyric acid              
Kinetin              
MES (free acid)              
myo-Inositol              
alpha-Naphthaleneacetic acid              
Nictotinic acid (free acid)              
Peptone              
Pyridoxine • HCl              
Sucrose              
Thiamine • HCl              
  M 5524 M 0654 M 0529 M 0153 M 2909 M 8900 M 8280
Grams of powder to prepare 1 L 4.3 n/a n/a 2.2 2.7 2.6 4.4
pH 1 0.5 at room temperature 3.9 4.3 3.2 3.9 3.9 3.9 4.1
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Media including Organics

   
M 6899
M 5519
M 0404
M 9274
M 7024 		 
Murashige and Skoog basal salts with minimal organics
Murashige and Skoog basal medium
Murashige and Skoog basal medium with Gamborg's vitamins
Murashige and Skoog basal medium with sucrose and agar
Murashige and Skoog shoot multiplication medium A
 
Component (mg/L) M 6899 M 5519 M 0404 M 9274 M 7024
Aluminum chloride • 6H2O          
Ammonium nitrate 1650.0 1650.0 1650.0 1650.0 1650.0
Ammonium phosphate monobasic          
Ammonium sulfate          
Boric acid 6.2 6.2 6.2 6.2 6.2
Calcium chloride anhydrous 332.2 332.2 332.2 332.2 332.2
Calcium nitrate          
Calcium phosphate tribasic          
Cobalt chloride • 6H2O 0.025 0.025 0.025 0.025 0.025
Cupric sulfate • 5H2O 0.025 0.025 0.025 0.025 0.025
Na2-EDTA 37.26 37.26 37.26 37.26 37.26
ZnNa2-EDTA          
Ferric chloride • 6H2O          
Ferric citrate          
Ferric sulfate          
Ferric tartrate • 2H2O          
Ferrous sulfate • 7H2O 27.8 27.8 27.8 27.8 27.8
Magnesium sulfate 180.7 180.7 180.7 180.7 180.7
Manganese chloride • 4H2O          
Manganese sulfate • H2O 16.9 16.9 16.9 16.9 16.9
Molybdenum trioxide          
Molybdic acid (sodium salt) • 2H2O 0.25 0.25 0.25 0.25 0.25
Nickel chloride • 6H2O          
Nickel sulfate • 6H2O          
Potassium chloride          
Potassium iodide 0.83 0.83 0.83 0.83 0.83
Potassium nitrate 1900.0 1900.0 1900.0 1900.0 1900.0
Potassium phosphate monobasic 170.0 170.0 170.0 170.0 170.0
Potassium sulfate          
Sodium nitrate          
Sodium phosphate monobasic         148.0
Sodium sulfate          
Zinc nitrate • 6H2O          
Zinc sulfate • 7H2O 8.6 8.6 8.6 8.6 8.6
Organics (mg/L) M 6899 M 5519 M 0404 M 9274 M 7024
Activated charcoal          
Adenine hemisulfate         80.0
Agar       8000.0  
6-Benzylaminopurine (BA)          
D-Biotin          
6gamma-gamma-
Dimethylallylaminopurine		         30.0
Glycine (free base)   2.0   2.0  
Indole-3-acetic acid         0.3
Indole-3-butyric acid          
Kinetin          
MES (free acid)          
myo-Inositol 100.0 100.0 100.0 100.0 100.0
alpha-Naphthaleneacetic acid          
Nictotinic acid (free acid)   0.5 1.0 0.5  
Peptone          
Pyridoxine • HCl   0.5 1.0 0.5  
Sucrose       30,000 30,000
Thiamine • HCl 0.4 0.1 10.0 0.1 0.4
  M 6899 M 5519 M 0404 M 9274 M 7024
Grams of powder to prepare 1 L 4.4 4.4 4.4 42.4 34.7
pH 1 0.5 at room temperature 3.9 3.9 3.8 5.0 3.9
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