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Life Science > Molecular Biology > Plant Biotechnology > Tissue Culture Protocols > Orchid Culture Media
Plant Tissue Culture Protocols

Orchid Culture Media

 
   
K 4003
K 4128
P 0931
P 1056
P 6668
P 6793
M 5524
 
Knudson C modified orchid medium
Knudson C orchid medium, morel modification
Phytamax orchid maintenance medium without charcoal
Phytamax orchid medium with charcoal and banana powder
Phytamax orchid maintenance medium
Phytamax orchid multiplication medium
Murashige and Skoog basal salt mixture (MS)
  (listed for purposes of comparison only)
 
Component (mg/L) K 4003 K 4128 P 0931 P 1056 P 6668 P 6793 M 5524*
Aluminum chloride • 6H2O              
Ammonium nitrate   500.0 825.0 825.0 825.0 825.0 1650.0
Ammonium phosphate monobasic              
Ammonium sulfate 500.0 500.0          
Boric acid 0.056   3.1 3.1 3.1 3.1 6.2
Calcium chloride anhydrous     166.0 166.0 166.0 166.0 332.2
Calcium nitrate 694.4 347.2          
Calcium phosphate tribasic              
Cobalt chloride • 6H2O     0.0125 0.0125 0.0125 0.0125 0.025
Cupric sulfate • 5H2O 0.0624   0.0125 0.0125 0.0125 0.0125 0.025
Na2-EDTA     37.24 37.24 37.24 37.24 37.26
ZnNa2-EDTA              
Ferric chloride • 6H2O              
Ferric citrate              
Ferric sulfate              
Ferric tartrate • 2H2O              
Ferrous sulfate • 7H2O 25.0 25.0 27.85 27.85 27.85 27.85 27.8
Magnesium sulfate 122.125 122.125 90.35 90.35 90.35 90.35 180.7
Manganese chloride • 4H2O              
Manganese sulfate • H2O 5.682 5.682 8.45 8.45 8.45 8.45 16.9
Molybdenum trioxide 0.016            
Molybdic acid (sodium salt) • 2H2O     0.125 0.125 0.125 0.125 0.25
Nickel chloride • 6H2O              
Nickel sulfate • 6H2O              
Potassium chloride   250.0          
Potassium iodide     0.415 0.415 0.415 0.415 0.83
Potassium nitrate     950.0 950.0 950.0 950.0 1900.0
Potassium phosphate monobasic 250.0 250.0 85.0 85.0 85.0 85.0 170.0
Potassium sulfate              
Sodium nitrate              
Sodium phosphate monobasic              
Sodium sulfate              
Zinc nitrate • 6H2O              
Zinc sulfate • 7H2O 0.331   5.3 5.3 5.3 5.3 8.6
Organics (mg/L) K 4003 K 4128 P 0931 P 1056 P 6668 P 6793 M 5524*
Activated charcoal       2000.0 2000.0    
Adenine hemisulfate              
Agar              
Banana powder (50% malto-dextrin)       30,000      
6-Benzylaminopurine (BA)           2.0  
D-Biotin              
6gamma-gamma-
Dimethylallylaminopurine
             
Glycine (free base)              
Indole-3-acetic acid              
Indole-3-butyric acid              
Kinetin              
MES (free acid)     1000.0 1000.0 1000.0 1000.0  
myo-Inositol     100.0 100.0 100.0 100.0  
alpha-Naphthaleneacetic acid           0.5  
Nictotinic acid (free acid)     1.0 1.0 1.0 0.5  
Peptone     2000.0 2000.0 2000.0 2000.0  
Pyridoxine • HCl     1.0 1.0 1.0 0.5  
Sucrose 20,000 20,000 20,000 20,000 20,000 20,000  
Thiamine • HCl     10.0 10.0 10.0 1.0  
  K 4003 K 4128 P 0931 P 1056 P 6668 P 6793 M 5524*
Grams of powder to prepare 1 L 21.6 22.0 25.3 57.3 27.3 25.3 4.3
pH ± 0.5 at room temperature 4.7 4.8 5.0 5.2 5.4 5.0 3.9

*Composition for M 5524, Murashige and Skoog Basal Salt Mixture, listed for purposes of
  comparison only.


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