DNA isolated from archived tissue is often of poor quality making it unsuitable for most commercially available whole genome amplification kits that require high quality starting material. The flexibility of the GenomePlex technology enables the use of compromised starting material, such as DNA isolated from FFPE tissue, for whole genome amplification. Sigma offers products for the purification of DNA from FFPE tissue (G1N10) and subsequent whole genome amplification (WGA2). We also offer the GenomePlex Tissue WGA Kit (WGA5) which includes optimized reagents for tissue disruption and cell lysis eliminating the need for tedious organic extractions to remove excess paraffin or DNA purification prior to amplification.
Advances in Genetic Analyis of Archived Samples, addresses the recent advances in Whole Genome Amplification that have made it possible to gain access to sufficient quantities of DNA from the millions of archived samples to perform a number of downstream applications including QPCR, CGH microarray, STR analysis and SNP analysis.
