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Life Science > Molecular Biology > Whole Genome Amplification > Formalin Fixed Paraffin Embedded DNA Amplification
Whole Genome Amplification

Formalin Fixed Paraffin Embedded DNA Amplification

Too many samples? Now available: WGA and WTA Services

DNA isolated from archived tissue is often of poor quality making it unsuitable for most commercially available whole genome amplification kits that require high quality starting material. The flexibility of the GenomePlex technology enables the use of compromised starting material, such as DNA isolated from FFPE tissue, for whole genome amplification. Sigma offers products for the purification of DNA from FFPE tissue (G1N10) and subsequent whole genome amplification (WGA2). We also offer the GenomePlex Tissue WGA Kit (WGA5) which includes optimized reagents for tissue disruption and cell lysis eliminating the need for tedious organic extractions to remove excess paraffin or DNA purification prior to amplification.

White Papers

Technical Articles

Posters

Protocols

Extraction

Amplification

Reamplification and purification

FFPE Diagnostic Protocol (257 Kb PDF)

Publications:
Click on the title to view the abstract on PubMed.

Title Author / Journal
Evaluation of whole genome amplification protocols for array and oligonucleotide CGH. Hittelman A, et al.Diagn Mol Pathol. 2007 Dec; 16(4), 198-206.
Application of array CGH on archival formalin-fixed paraffin embedded tissues including small numbers of microdissected cells. Johnson, N. A. et al.
Lab. Invest. 86(9), 968-78 (2006).
Array CGH using whole genome amplification of fresh-frozen and formalin-fixed, paraffin-embedded tumor DNA. Little, S. E. et al.
Genomics Oct. 31, 2005.

Webcast:

Advances in Genetic Analysis of Archived Samples - Video Webcast
Advances in Genetic Analyis of Archived Samples, addresses the recent advances in Whole Genome Amplification that have made it possible to gain access to sufficient quantities of DNA from the millions of archived samples to perform a number of downstream applications including QPCR, CGH microarray, STR analysis and SNP analysis.

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